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ProteomicsMudPIT analyses of the proteins co-purified with FLAG-HA Non-Stop FLAG-IPed from Drosophila melanogaster S2 cells
Protein Complexes Purification- Drosophila S2 cells were stably-transfected with pRmHa3-based inducible expression vectors to produce epitope-tagged Non-stop-2xFLAG-2xHA (Non-stop-FH). Cells stably transfected with empty vector served as negative control. Protein complexes containing Non-stop were p...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2018-07-17 | MSV000082625 | MassIVE
ProteomicsTandem affinity purification and Multidimensional Protein Identification Technology to identify USP44 interacting proteins
Nuclear extracts were isolated from 293T cells that stably express USP44 fused with N-terminal FLAG and HA affinity tags (FH-USP44), and then analyzed by tandem affinity purification (TAP) followed by Multidimensional Protein Identification Technology (MudPIT) to uncover USP44 associated proteins. T...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000080144 | MassIVE
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