The precipitated proteins from whole cells were resuspended in 50 μl of 500 mM ammonium bicarbonate containing 8 M urea, and reduced with 5 μl of 200 mM DTT. The samples were incubated at 90°C for 30 min and cooled to room temperature. Free cysteine residues were alkylated with 10 μl of 200 mM iodoa...
ORGANISM(S): Mus Musculus (mouse)
