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ProteomicsSurfactant-assisted one-pot sample preparation for label-free single-cell proteomics
Large numbers of cells are generally required for quantitative global proteome profiling due to the significant surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic profiling impossible...
ORGANISM(S): Homo Sapiens 
2021-03-11 | PXD022827 | panorama
ProteomicsMm_CSF
Characterization of individual mouse cerebrospinal fluid proteomes
ORGANISM(S): Mus Musculus 
2014-12-31 | PAe005205 | PeptideAtlas
ProteomicsReproducible Workflow for Multiplexed Deep-Scale Proteome and Phosphoproteome Analysis of Tumor Tissues by Liquid Chromatography-Mass Spectrometry

Mertins P, Tang LC, Krug K, Clark DJ, Gritsenko MA, Chen L, Clauser KR, Clauss TR, Shah P, Gillette MA, et al., Nat Protoc. 2018 Jul 9. doi: 10.1038/s41596-018-0006-9

Here we present an optimized workflow for gl...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2019-08-30 | MSV000084265 | MassIVE
ProteomicsAn intelligent Boosting to Amplify Signal with Isobaric Labeling (iBASIL) strategy for precise quantitative single-cell proteomics
Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective proce...
ORGANISM(S): Homo Sapiens (human) 
2020-03-05 | PXD016057 | JPOST Repository
ProteomicsAn improved Boosting to Amplify Signal with Isobaric Labeling (iBASIL) strategy for precise quantitative single-cell proteomics
Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective proce...
ORGANISM(S): Homo Sapiens (human) 
2020-03-05 | PXD017626 | JPOST Repository
ProteomicsA streamlined tandem tip-based workflow for sensitive nanoscale phosphoproteomics
Effective nanoscale phosphoproteome analysis remains a daunting task, primarily due to significant sample loss associated with non-specific surface adsorption during phosphopeptide enrichment and other sample processing steps. To address this issue, we have developed a tandem tip-based C18-IMAC-C18 ...
ORGANISM(S): Homo Sapiens (human) 
2023-01-27 | PXD032019 | JPOST Repository
MetabolomicsSPE-IMS-MS: An automated platform for sub-sixty second surveillance of endogenous metabolites and xenobiotics in biofluids
Characterization of endogenous metabolites and xenobiotics is essential to deconvoluting the genetic and environmental causes of disease. However, surveillance of chemical exposure and disease-related changes in large cohorts requires an analytical platform that offers rapid measurement, high sensit...
2017-02-14 | MTBLS427 | MetaboLights
ProteomicsHanging-droplet sample preparation improves coverage for spatially-resolved proteomics
We developed a hanging drop (HD) method to improve the sample recovery for spatial proteomics by placing the nanowell chip in an upside-down direction during droplet-based sample preparation. Compared with the commonly-used sitting-drop (SD) method, the HD method can keep the tissue voxel away from ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-04-21 | MSV000089295 | MassIVE
ProteomicsIschemiaOC_Proteome_PNNL
Human Ovarian Cancer, iTRAQ
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe004951 | PeptideAtlas
ProteomicsTime-Dependent Proteome Study: Human ovarian cancer tumor samples (0, 5, 30, 60 min)

The objective of the Time-Dependent Proteome Study, was to evaluate changes in the proteome and phosphoproteome when there is a delay in freezing tissues following sample (tumor) excision. The biospecimens used are from patient-derived ovarian cancer tumors.

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-11-05 | MSV000083107 | MassIVE
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