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Three independent biological replicates of HCT116 cells transiently transfected with FLAG-tagged RPS19 expression vectors or empty vectors were fixed with 0.1% formaldehyde methanol-free (Pierce) and then lysed on ice in 1 ml of HEPES-RIPA2 buffer (20 mM HEPES-NaOH pH7.5, 1 mM EGTA, 1 mM MgCl2, 150 ...
ORGANISM(S): Homo Sapiens (human) 
HCT116 cells transiently transfected with FLAG-tagged RPS7, RPS17, or RPS19 expression vectors or empty vectors were fixed with 0.1% formaldehyde methanol-free (Pierce) and then lysed on ice in 1 ml of HEPES-RIPA2 buffer (20 mM HEPES-NaOH pH7.5, 1 mM EGTA, 1 mM MgCl2, 150 mM NaCl, 0.25% Na-deoxychol...
ORGANISM(S): Homo Sapiens (human) 
A koji mold Aspergillus kawachii is used for making a Japanese distilled spirit, shochu. During making shochu, A. kawachii is grown in a solid-state culture comprised of steamed grains such as rice or barley, termed koji, to convert the starch to glucose and also to produce citric acid. During this ...
ORGANISM(S): Aspergillus kawachii 
MS/MS spectra assigned to peptides with more than six amino acids were selected from our database and converted into MRM transitions. Overlapping ions with a difference in mass/charge ratio (m/z) of 400 were preferentially selected
ORGANISM(S): Homo Sapiens (human) 
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
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