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ProteomicsLFQ of GFP-Trap IP-MS of BmN-4 cells expressing BmMasc-GFP
BmMasc-GFP, BmMascNLS-GFP, or GFP was transiently expressed in BmN-4 cells seeded in 10 cm-diameter culture dishes. At three days post transfection, the cells were fixed with 0.1% formaldehyde, washed, and lysed on ice for 10 min in 1 mL of RIPA buffer (20 mM HEPES-KOH (pH 7.5), 1 mM EGTA, 1 mM MgCl...
ORGANISM(S): Cellular Organisms 
2025-05-31 | PXD060754 | JPOST Repository
ProteomicsLFQ of immunoprecipitates with GFP-Trap from GFP-, OfMasc-GFP-, or Del1-OfMasc-GFP-transfected cells
OfMasc-GFP derivatives or GFP were transiently expressed in OfTC cells. At 2 days post transfection, OfTC cells were treated with 100 nM Bortezomib for 1 day. The OfT1C cells were then fixed with 0.1% formaldehyde, and the fixation was quenched with 300 mM glycine. The cells were lysed on ice in 1 m...
ORGANISM(S): Cellular Organisms 
2022-10-04 | PXD034837 | JPOST Repository
MetabolomicsNiacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma (Cell NAMPTi treatment)

NAMPT plays a major role in NAD biosynthesis in many cancers and is an attractive potential cancer target. However, factors dictating therapeutic efficacy of NAMPT inhibitors (NAMPTi) are unclear. We report that neuroendocrine phenotypes predict lung and prostate carcinoma vulnerability to NAMPTi...

2023-10-10 | MTBLS7251 | MetaboLights
MetabolomicsHigh-Precision Automated Workflow for Urinary Untargeted Metabolomic Epidemiology
Urine is a non-invasive biofluid that is rich in polar metabolites and well-suited for metabolomic epidemiology. However, due to individual variability in health and hydration status, the physiological concentration of urine can differ >15-fold, which can pose major challenges in untargeted LC-MS me...
2021-06-10 | MTBLS2295 | MetaboLights
MetabolomicsNiacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma (Mouse NAD targeted time-course)

NAMPT plays a major role in NAD biosynthesis in many cancers and is an attractive potential cancer target. However, factors dictating therapeutic efficacy of NAMPT inhibitors (NAMPTi) are unclear. We report that neuroendocrine phenotypes predict lung and prostate carcinoma vulnerability to NAMPTi...

2023-10-10 | MTBLS7253 | MetaboLights
MetabolomicsNiacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma (Mouse NAMPT targeted therapy)

NAMPT plays a major role in NAD biosynthesis in many cancers and is an attractive potential cancer target. However, factors dictating therapeutic efficacy of NAMPT inhibitors (NAMPTi) are unclear. We report that neuroendocrine phenotypes predict lung and prostate carcinoma vulnerability to NAMPTi...

2023-10-10 | MTBLS7252 | MetaboLights
ProteomicsWhole-cell proteome of Thermosulfidibacter takaii strain ABI70S6
Identification of the proteins from tryptic digests of Thermosulfidibacter takaii strain ABI70S6 whole-cell proteome followed by nanoLC/OrbitrapFusion MS analysis.
ORGANISM(S): Cellular Organisms 
2017-11-24 | PXD007930 | JPOST Repository
UnknownGenetic insights into ossification of the posterior longitudinal ligament of the spine.
Not available
S-EPMC10353864 | biostudies-literature
ProteomicsProteome analysis of brainstem organoids from human pluripotent stem cells
Brain organoids derived from human pluripotent stem cells recapitulate the course of human brain development. We applied proteomic analysis to human brainstem organoids which were developed by a novel method.
ORGANISM(S): Homo Sapiens (human) 
2020-05-01 | PXD016807 | JPOST Repository
UnknownSARS-CoV-2 vaccination modelling for safe surgery to save lives: data from an international prospective cohort study.
Not available
S-EPMC7995808 | biostudies-literature
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