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ProteomicsOne-step N-terminomics based on isolation of protein N-terminal peptides from LysargiNase digests by tip-based strong cation exchange chromatography
Large-scale isolation of protein N-terminal peptides from LysargiNase digests was achieved by one-step strong cation exchange chromatography with disposable and parallel-processable pipette-tip columns. Novel cleavage sites for signal or transit peptides and non-canonical translation initiation site...
ORGANISM(S): Homo Sapiens (human) 
2024-08-13 | PXD048917 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – IP input
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-02-09 | PXD024079 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – CX4945 treatment
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-02-09 | PXD024080 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – Nt-acetylation analysis
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-02-09 | PXD024081 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - mouse primary neuron DIV5 vs DIV14
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2022-05-28 | PXD026346 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – peptide pulldown
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-05-28 | PXD026349 | JPOST Repository
ProteomicsNovel roles of small extracellular vesicles in regulating the quiescence and proliferation of neural stem cells
We analyzed protein cargos of NSC sEVs from proliferating, resting and reactivating status by proteomics approaches. Our results revealed specific functional clusters of pathways in gene ontology annotations of differentially expressed proteins in three sources of exosomes. Furthermore, we showed th...
ORGANISM(S): Mus Musculus (mouse) 
2021-10-27 | PXD027651 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - pSNAP evaluation
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2023-02-07 | PXD024078 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - mouse primary neuron
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2023-02-07 | PXD024104 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – LPS stimulation to RAW264.7 macrophages
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2023-02-07 | PXD031475 | JPOST Repository
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