CD4 SP thymocytes were sorted and unstimulated or stimulated with α-CD3 mAb and α-CD28 mAb for 16 hours. Cells were washed with PBS and lysed in 150 μl of 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrif...
ORGANISM(S): Mus Musculus (mouse)
