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ProteomicsDe novo protein identification in mammalian sperm using high-resolution in situ cryo-electron tomography
Understanding molecular mechanisms of cellular pathways regularly requires knowledge of the identities of participating proteins, their cellular localization and their 3D structures. Contemporary workflows typically require multiple techniques to identify target proteins, track their localization us...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-21 | PXD036885 | Pride
Global protein abundance In situ cryoet Mouse sperm axoneme Sperm flagella Visual proteomics 
UnknownCfap97d1 is important for flagellar axoneme maintenance and male mouse fertility.
Not available
S-EPMC7444823 | biostudies-literature
UnknownTektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella.
Not available
S-EPMC10917850 | biostudies-literature
ProteomicsTesticular Proteomics of PCM1 knockdown mice
Loss of pcm1 in mice disrupts the centrosome remodel and flagellar formation during spermatogenesis.
ORGANISM(S): Mus Musculus 
PXD051164 |
UnknownPhosphorylation of mouse sperm axoneme central apparatus protein SPAG16L by a testis-specific kinase, TSSK2.
Not available
S-EPMC2660405 | biostudies-literature
UnknownFlagellar energetics from high-resolution imaging of beating patterns in tethered mouse sperm.
Not available
S-EPMC8159377 | biostudies-literature
ProteomicsChaperonin TRiC bridges radial spokes for folding proteins locally translated in mammalian sperm flagella
As animals evolved from external to internal fertilization, sperm flagella, which once transiently propelled sperm in water to reach nearby eggs, developed to beat for days or even longer within the female reproductive tract. How flagella were remodeled accordingly remains unclear. Unlike externally...
ORGANISM(S): Bos Taurus 
PXD067693 |
UnknownAdenine nucleotide metabolism and a role for AMP in modulating flagellar waveforms in mouse sperm.
Not available
S-EPMC4094002 | biostudies-literature
TranscriptomicsNext Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes at post natal day 21 [RNA-Seq P21]
Purpose: This study was carried out to determine the consequences of the Rfx2-/- genotype on spermatogenesis in the mouse Methods: RNA was extracted from decapsulated testes of 21 day old mixed background mice of either genotype. Deep sequencing was used to determine quantitative expression of the g...
ORGANISM(S): Mus musculus 
2015-11-02 | E-GEOD-68284 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes at post natal day 30 [RNA-Seq P30]
Purpose: This study was carried out to determine the consequences of the Rfx2-/- genotype on spermatogenesis in the mouse Methods: RNA was extracted from decapsulated testes of 29-30 day old mixed background mice of either genotype. Deep sequencing was used to determine quantitative expression of th...
ORGANISM(S): Mus musculus 
2015-11-02 | E-GEOD-68283 | biostudies-arrayexpress
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