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GenomicsDynamics of Sox2 occupancy during the differentiation of embryonic stem cells into trophoblast stem cells.
To understand the mechanism underlying the versatility in transcriptional regulation by Sox2, we compared genome-wide binding sites of Sox2 in embryonic stem (ES) cells and trophoblast stem (TS) cells by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). A tetracycline-...
ORGANISM(S): Mus musculus 
2013-10-17 | E-GEOD-28453 | biostudies-arrayexpress
GenomicsSox2-regulatory networks in embryonic and trophoblast stem cells.
Sox2 is a pleiotropic transcription factor that regulates self-renewal and differentiation capacity in different types of stem cells, raising the possibility that it regulates similar transcriptional programs controlling common stemness. Embryonic stem (ES) cells and trophoblast stem (TS) cells are ...
ORGANISM(S): Mus musculus 
2013-10-17 | E-GEOD-28455 | biostudies-arrayexpress
TranscriptomicsConditional knockout of Sox2 in embryonic and trophoblast stem cells.
To compare the transcriptional networks governed by Sox2 in embryonic stem (ES) cells and trophoblast stem (TS) cells, we performed whole-genome expression analysis after tetracycline (Tet)-induced knockout of Sox2 in each cell type. A Tet-inducible Sox2 knockout ES cell line 2TS22C or its derivativ...
ORGANISM(S): Mus musculus 
2013-10-17 | E-GEOD-28451 | biostudies-arrayexpress
TranscriptomicsExpression and ChIP-seq analyses of embryonic stem cells, extraembryonic endoderm stem cells, and trophoblast stem cells
Bivalent histone domains have been proposed to contribute to pluripotency in embryonic stem cells, suggesting an epigenetic mechanism may regulate stem cell behavior in general. Here we compare histone modifications in two other stem cells derived from the blastocyst. We show that extraembryonic ste...
ORGANISM(S): Mus musculus 
2010-05-16 | E-GEOD-15519 | biostudies-arrayexpress
TranscriptomicsInduction of trophoblast stem cells from embryonic stem cells by forced repression of Oct3/4.
To characterize the transdifferentiation of embryonic stem (ES) cells into trophoblast stem (TS) cells triggered by forced repression of Oct3/4, we performed whole-genome expression analysis after tetracycline (Tet)-induced knockout of Oct3/4. A Tet-inducible Oct3/4 knockout ES cell line ZHBTc4 was ...
ORGANISM(S): Mus musculus 
2013-10-17 | E-GEOD-28452 | biostudies-arrayexpress
GenomicsGenome-wide mapping of Eset-binding sites and H3K9me3 state in mouse embryonic stem cells
The histone H3 lysine 9 (H3K9) methyltransferase Eset is an epigenetic regulator critical for the development of the inner cell mass (ICM). Although ICM-derived embryonic stem (ES) cells are normally unable to contribute to the trophectoderm (TE) in blastocysts, we find that depletion of Eset by shR...
ORGANISM(S): Mus musculus 
2009-11-04 | E-GEOD-17642 | biostudies-arrayexpress
GenomicsConversion of Fibroblasts into Functional Trophoblast Stem-like Cells
Here we demonstrate the generation of stable induced trophoblast stem cells (iTSCs) from fibroblasts by the transient expression of Gata3, Eomes and Tfap2c. Transcriptome and methylome analyses and functional assays such as hemorrhagic lesion formation and placenta contribution suggested a high deg...
ORGANISM(S): Mus musculus 
2016-07-05 | E-GEOD-70234 | biostudies-arrayexpress
TranscriptomicsGata3 acts alongside Cdx2 to promote trophoblast gene expression downstream of Tead4 during mouse development
The first lineage decisions during mouse development lead to establishment of embryonic and extraembryonic tissues. The transcription factor Cdx2 plays a central role by repressing pluripotency genes, such as Oct4 and promoting trophoblast fate at the blastocyst stage. Here we show that the transcri...
ORGANISM(S): Mus musculus 
2010-02-21 | E-GEOD-12999 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of placental trophoblast stem cells time dependent response to hyperosmolar stress
Stress induces undifferentiated stem cells to differentiate in a way that looks like normal differentiation; We used hyperosmolar stress as a generic, energy-depleting stressor and tested for the global changes in the transcriptional response of placental trophoblast stem cells Experiment Overall De...
ORGANISM(S): Mus musculus 
2008-11-13 | E-GEOD-9519 | biostudies-arrayexpress
GenomicsCTCF binding patterns in mouse trophoblast stem cells
This Series reports data from a CTCF ChIP-Seq experiment performed in F1-hybrid mouse trophoblast stem cells (TSCs). The data are part of a larger study examining inactive X gene expression and chromatin states, reported as GEO Series GSE39406. Included for this dataset are FASTQ files, BED alignmen...
ORGANISM(S): Mus musculus 
2012-09-08 | E-GEOD-40667 | biostudies-arrayexpress
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