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ProteomicsComparison of different sample preparation protocols reveals lysis buffer specific extraction biases in gram-negative bacteria and human cells
We evaluated different in-solution and FASP-based sample preparation strategies for absolute protein quantification. Label-free quantification (LFQ) was employed to compare different sample preparation strategies in the bacterium Pseudomonas aeruginosa and human embryonic kidney cells (HEK) and note...
ORGANISM(S): Homo sapiens (Human) Pseudomonas aeruginosa PAO1 
2015-10-06 | PXD001511 | Pride
Hek293 Pseudomonas aeruginosa 
ProteomicsBarley leaf sample preparation
Barley is an important cereal crop all over the world. Detailed molecular characterization of barley provides the basis for development of improved cultivars, stress and drought-resistant plants. We here present an LC-MS/MS-based proteomics study of barley leaf aimed at optimization of methods to ac...
ORGANISM(S): Hordeum vulgare (Barley) 
2018-09-03 | PXD008938 | Pride
Mass spectrometer Hordeum vulgare In solution digestion Sample preparation Sodium deoxycholate 
ProteomicsIsobaric peptide labeling on digital microfluidics for quantitative proteomics of low cell numbers is facilitated by low critical micellar concentration detergents
We developed protocols for isobaric TMT labeling on digital microfluidics (DMF) devices enabling the quantitative proteome analysis of approximately 25 mammalian cells per channel by subsequent bottom-up proteome analysis by LC-MS. This comprised identification of a compatible detergent for digestio...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD023226 | Pride
Digital microfluidics Detergents Cmc Tmt labeling Sp3 
UnknownMass spectrometry of intact membrane protein complexes.
Not available
S-EPMC4058633 | biostudies-literature
UnknownRapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites.
Not available
S-EPMC5851482 | biostudies-literature
ProteomicsProteome profiling of FF and FFPE microdissected human kidney tissue
Aim of the project was to evaluate several MS-comaptible detergents for processing fresh frozen (FF) and formalin fixed paraffin embedded (FFPE) microdissected human kidney tissue. Here we have evaluated sensitivity of the methods and their applicability on FF and FFPE tissues, as well as investigat...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD020793 | Pride
Ff Ms-compatible protocols Mass spectrometry Ffpe Kidney 
UnknownA method for filamentous fungal growth and sample preparation aimed at more consistent MALDI-TOF MS spectra despite variations in growth rates and/or incubation times.
Not available
S-EPMC7200990 | biostudies-literature
UnknownSimultaneous transcranial magnetic stimulation and single-neuron recording in alert non-human primates.
Not available
S-EPMC4115015 | biostudies-literature
ProteomicsEfficiency of the purification protocols on complex matrices: biofilm, mouse liver and soil
In gel-free experimental workflows, protein purification often starts with a precipitation stage using trichloroacetic acid (TCA). In presence of TCA, proteins precipitate in a stable molten globule state making the pellet difficultto solubilize in aqueous buffer for proteolytic digestion and MS ana...
ORGANISM(S): Mus musculus (Mouse) Porphyra purpurea Trichoderma cf. viride DIS 217I Synechocystis sp. CCALA 700 Microchaete diplosiphon CCALA 811 
2017-11-30 | PXD008110 | Pride
Mouse liver Biofilm Soil Lc-ms/ms 
UnknownDetection and mapping of illicit drugs and their metabolites in fingermarks by MALDI MS and compatibility with forensic techniques.
Not available
S-EPMC4484357 | biostudies-literature
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