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Absolute quantification of clinical biomarkers by mass spectrometry (MS) has been challenged due to low sample-throughput of current multiple reaction monitoring (MRM) methods. For this problem to be overcome, in this work, a novel high-sample-throughput multiple reaction monitoring mass spectrometr...
ORGANISM(S): Homo Sapiens 
2020-02-23 | PXD040708 |
To characterize the changes of phosphorylation signaling cascades induced by LAT1 inhibition in MIA PaCa-2 cells treated with LAT1 specific inhibitor JPH203, immobilized metal affinity chromatography and liquid chromatography-tandem mass spectrometry with MS2-based quantitation of tandem mass tag we...
ORGANISM(S): Homo Sapiens (human) 
In this project we use MS2-based 6-plex TMT quantitation to compare phosphorylation site abundances across several stages of the Plasmodium falciparum life cycle in wild-type Plasmodium and in a strain whose PK7 has been knocked out. See Pease et al. (2018) J. Proteome Res. for details.
ORGANISM(S): Homo sapiens (Human) Plasmodium falciparum 
2018-10-04 | PXD009465 | Pride

The sample to be tested was fully ground into powder in the grinder, 50 mg of the sample was freeze-dried, 1000 μL of the extraction solution containing the inner target was added (methanol:acetonitrile:water, 2:2:1, v/v/v, internal standard concentration 20 mg/L) and vortex mixed for 30 s; then ...

2024-07-12 | MTBLS10002 | MetaboLights
The NFE2L2/NRF2 oncogene and transcription factor drives a gene expression program that promotes cancer progression, metabolic reprogramming, immune evasion and chemoradiation resistance. Patient stratification by NRF2 activity may guide treatment decisions to improve outcome. Here, we developed a m...
ORGANISM(S): Homo sapiens (Human) 
2023-06-08 | PXD041162 | Pride
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