FLAG-VISTA expressed in Y-MESO-14 or NCI-H2373 cells was immunoprecipitated with anti-FLAG antibody. Parental cells were used for control samples. Immunoprecipitates were analyzed by LC-MS/MS.
The kinase assay was performed using IP products that combined the lysates of human cultured cells ectopically expressing five NS proteins, followed by identification of the proteins from the third broad band group extracted from the gel.
