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TranscriptomicsLin28b expression distinguishes fetal versus adult lymphopoiesis
This SuperSeries is composed of the following subset Series: GSE35081: miRNA profiling of double-positive thymocytes transduced with a retrovirus encoding Lin28a compared to untransduced controls GSE35107: microRNA profiling of fetal liver and adult bone marrow pro-B cells Refer to individual Series
ORGANISM(S): Mus musculus 
2012-02-17 | E-GEOD-35214 | biostudies-arrayexpress
TranscriptomicsmiRNA profiling of double-positive thymocytes transduced with a retrovirus encoding Lin28a compared to untransduced controls
We used the NanoString mouse nCounter miRNA expression platform to compare the miRNA expression in double-positive (DP) thymocytes that developed in the presence (GFP+) or absence (GFP-ve) of ectopic mLin28 expression. Transduced (GFP+) and untransduced (GFP-ve) CD4+ CD8+ CD3- thymocytes were FACS s...
ORGANISM(S): Mus musculus 
2012-02-17 | E-GEOD-35081 | biostudies-arrayexpress
TranscriptomicsTranscriptome of double-positive thymocyte precursors transduced with a retrovirus encoding Lin28a
We performed paired-end RNA-seq to compare the transcriptome of DP thymocytes that ectopically express Lin28 in vivo versus untransduced (GFP-ve) DP thymocytes. Transduced (GFP+) and untransduced (GFP-) CD4+ CD8+ CD3- thymocytes were sorted and pooled from three recipients of hematopoietic stem and ...
ORGANISM(S): Mus musculus 
2012-02-17 | E-GEOD-34854 | biostudies-arrayexpress
TranscriptomicsExpression and regulation of lincRNAs during T cell development and differentiation
Although lincRNAs are implicated in regulating gene expression in various tissues, little is known about lincRNA transcriptomes in the T cell lineages. Here we identify 1,524 lincRNAs in 42 T cell samples from early T cell progenitors to terminally differentiated T helper subsets. Our analysis revea...
ORGANISM(S): Mus musculus 
2013-09-23 | E-GEOD-48138 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse Abl pre-B cell lines with conditional deletion of dicer
Abelson virus (v-Abl)-transformed pre-B cell lines from BM of dcrfl/fl; R26R-yfp (4470; 4475) and dcrfl/+; R26R-yfp mice (4483) are treated with a transducible Cre protein (Tat-Cre) to induce deletion of the dicer-1 gene in vitro. Cre activity is conveniently monitored by concomitant expression of Y...
ORGANISM(S): Mus musculus 
2008-03-08 | E-MEXP-1455 | biostudies-arrayexpress
TranscriptomicsExpression profiling of DT40 chicken B cell line by RNA-seq
A single replicate of exponentially growing DT40 CL18 chicken B lymphoma cells were harvested and extracted RNA was subjected to Illumina GAIIx paired-end sequencing to determine global gene expression. Single replicate RNA-seq expression analysis of DT40 cells.
ORGANISM(S): Gallus gallus 
2015-09-04 | E-GEOD-58766 | biostudies-arrayexpress
TranscriptomicsRegulation of microRNA Expression and Abundance during Lymphopoiesis
Although the cellular concentration of miRNAs is critical to their function, how miRNA expression and abundance are regulated during ontogeny is unclear. We used deep-sequencing to characterize the microRNome from most developing lymphocytes, various hematopoietic cell lineages, and representative m...
ORGANISM(S): Mus musculus 
2010-06-07 | E-GEOD-21630 | biostudies-arrayexpress
GenomicsJarid2, a Polycomb member and target of miR-155, regulates the fates of Th17 and Treg cells
Genome-wide analysis of Jarid2, Suz12, and c-Maf binding and H3K27me3 profiling in miR-155 KO and WT Th17 performed by ChIP-seq. We found that Jarid2 and c-Maf is differentially expressed in absence of miR-155 and they compete for binding to the Il22 promoter. We highlight targets of Jarid2 and Suz...
ORGANISM(S): Mus musculus 
2014-06-01 | E-GEOD-47528 | biostudies-arrayexpress
TranscriptomicsmicroRNA expression in human tonsillar B cell populations
small RNA profiles of 6 human tonsillar B cell populatios (naive B cells, pre-germinal center B cells, centrocytes, centroblasts, memory B cells, and plasma cells) were determined by deep sequencing. These samples were compared to mouse developing lymphocytes, various hematopoietic cell lineages, a...
ORGANISM(S): Homo sapiens 
2010-07-23 | E-GEOD-23090 | biostudies-arrayexpress
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