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ProteomicsRapid and Label-Free Structural Proteomics by One-Step Swift Trypsin LiP-MS
We developed a rapid, surface-targeted limited proteolysis workflow, Swift Trypsin LiP-MS (STLiP-MS), to profile proteome-wide structural changes under near-native conditions. STLiP-MS uses a trypsin-immobilized spin column and centrifugation-controlled contact to achieve instantaneous, highly repro...
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2026-01-05 | PXD069771 | JPOST Repository
ProteomicsTurboID-mediated proximal labeling of Drosophila BubR1 and its cleavage variants
V5-TurboID-tagged Drosophila BubR1 and its cleavage variants were transiently expressed in Drosophila S2 cells in two biological replicates. Cells were treated with 500 µM biotin for 30 min for protein biotinylation. Biotinylated protein-containing lysate was subjected to FG-NeutrAvidin beads purifi...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2023-05-11 | PXD033797 | JPOST Repository
ProteomicsDIA-MS based proteome analysis of HL-60 cells treated with new anti-cancer drug (TAS4464)
We investigated the protein change of HL-60 cells with TAS4464 treatment in time series (0 h, 1h, 4h, 8h, 16h, 24h) by overlapping DIA-MS based proteome analysis. Spectral library was created by analyzing pooled samples by five gas-phase fractionated overlapping DIA-MS measurements.
ORGANISM(S): Homo Sapiens (human) 
2021-08-20 | PXD021012 | JPOST Repository
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