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ProteomicsThe Identification of Proteolytic Substrates of Calpain-5 with N-terminomics
Calpain-5/CAPN5 is a calcium-activated, non-lysosomal cysteine (thiol) protease. The substrate spectrum repertoire of CAPN5 is not known. Calpains catalyze limited proteolysis of their substrates, generating novel neo protein N-termini that correspond to internal residues of their nascent substrate ...
ORGANISM(S): Homo sapiens (Human) 
2025-07-21 | PXD064313 | Pride
Capn5 Protease Disease Tails N-terminomics Calpain-5 
ProteomicsN-terminomics/TAILS profiling of macrophages after chemical inhibition of legumain
We developed a legumain inhibitor and activtiy based probes. Murine RAW264.7 cells were subjected to N-terminomics and shotgun proteomics analysis.
ORGANISM(S): Mus musculus (Mouse) 
2019-09-05 | PXD015200 | Pride
Macrophage Protease Legumain 
ProteomicsFAIMS-enabled N-terminomics analysis reveals novel legumain substrates in murine spleen
Legumain is cysteine protease primarily localised to the endo-lysosomal system. Upregulated legumain activity is associated with inflammation, neurodegeneration, and tumorigenesis. Whilst inhibiting legumain in mouse models has demonstrated therapeutic benefit, the proteolytic mechanisms underpinnin...
ORGANISM(S): Homo sapiens (Human) 
2024-05-23 | PXD047733 | Pride
Faims Legumain Protease N-terminomics 
ProteomicsFAIMS-enabled N-terminomics analysis reveals novel legumain substrates in murine spleen
Legumain is cysteine protease primarily localised to the endo-lysosomal system. Upregulated legumain activity is associated with inflammation, neurodegeneration, and tumorigenesis. Whilst inhibiting legumain in mouse models has demonstrated therapeutic benefit, the proteolytic mechanisms underpinnin...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-23 | PXD047734 | Pride
Faims Legumain Protease N-terminomics 
ProteomicsN-terminomics Analysis of Mouse cultured cortical neuron during excitotoxicity
Excitotoxicity is a primary pathological process directing neuronal cell death in both acute neurological disorders and neurodegenerative diseases such as ischemic stroke and Alzheimer’s disease. We use mouse cultured cortical neuron treated with 100uM of Glutamate for a model of excitotoxicity and ...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-07 | PXD040648 | Pride
N-term Excitotoxicity 
ProteomicsN-terminomics for the identification of in-vitro substrates and cleavage site specificity of the SARS-CoV-2 main protease
The genome of coronaviruses, including the SARS-CoV-2, which causes the human pandemic COVID-19, encodes for two proteases, a papain like (PL) and the so-called Main protease (Mpro), also named 3CLpro or non-structural protein 5 (NSP5). Mpro is activated by autoproteolysis, and true to its name, is ...
ORGANISM(S): Homo sapiens (Human) 
2020-11-02 | PXD021406 | Pride
Sars-cov-2 Sars-cov N-terminomics Lcms Proteomics 
ProteomicsN-terminomics profiling of naive and inflamed murine colon reveals proteolytic signals of legumain
Legumain is a cysteine protease broadly associated with inflammation. It has recently been reported to cleave and activate protease-activated receptor 2 to provoke pain associated with oral cancer. Outside of gastric and colon cancer, little has been reported on the roles of legumain within the gast...
ORGANISM(S): Mus musculus (Mouse) 
2025-09-29 | PXD051470 | Pride
Protease N-terminomics Legumain Colon Colitis 
ProteomicsN-terminomics/TAILS profiling of proteases and their substrates in ulcerative colitis
Dysregulated protease activity is often implicated in the initiation of inflammation and immune cell recruitment in gastrointestinal inflammatory diseases. Using N-terminomics/TAILS (terminal amine isotopic labeling of substrates), we compared proteases, along with their substrates and inhibitors, b...
ORGANISM(S): Homo sapiens (Human) 
2019-09-08 | PXD014479 | Pride
Protease Biopsies Ulcerative colitis Tails 
ProteomicsReverse N-terminomics of SARS-CoV-2 PLpro in A549
Subtiligase based N-terminomics (Abu N-terminal tagging) of two A549 cell lysate replicates incubated with SARS-CoV-2 PLpro.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-08-12 | MSV000090125 | MassIVE
ProteomicsReverse N-terminomics of SARS-CoV-2 PLpro in Jurkat
Subtiligase based reverse N-terminomics (Abu N-terminal tagging) of two Jurkat cell lysate replicates incubated with SARS-CoV-2 PLpro.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-08-12 | MSV000090124 | MassIVE
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