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ProteomicsIEF fractionation OVCAR-5 cell line
Analysis of the ovarian cancer cell line OVCAR-5. A standard trypsin digest was carried out on the OVCAR-5 cell lysates which were then analysed in the un-fractionated and fractionated forms. Fractionation was completed using a peptide IEF separation method. All samples were analysed by nano-LC-ESI-...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD003547 | Pride
Ief fractionation lc-ms/ms ovarian cancer cell line 
UnknownExtinction of conditioned fear is better learned and recalled in the morning than in the evening.
Not available
S-EPMC3791331 | biostudies-literature
TranscriptomicsSilencing of AEBP1 in U87MG glial cells and Chip-chIP with AEBP1 antibody
AEBP1 has been identified as a transcriptional repressor playing a significant role in adipogenesis. Recently, we have found that AEBP1 is specifically upregulated in primary glioblastoma. We have used gene silencing to identify genes perturbed upon AEBP1 downregulation and ChIP-chip technique to i...
ORGANISM(S): Homo sapiens 
2012-04-29 | E-GEOD-18892 | biostudies-arrayexpress
MetabolomicsSulfadiazine Sodium Improves Dysfunctional Metabolomics in Toxoplasma gondii-infected Mice
Toxoplasma gondii is an obligate intracellular protozoan parasite that poses a severe threat to human health, especially those immune-compromised individuals. Sulfadiazine sodium (SDZ) is widely used for the treatment of human toxoplasmosis. However, systems biology researches on the therapeutic eff...
2019-06-18 | MTBLS852 | MetaboLights
TranscriptomicsUAP56 couples piRNA clusters to the perinuclear transposon silencing machinery [RNA-Seq and RIP-Seq]
The transposon silencing piRNAs are produced from precursors that are encoded by heterochromatic clusters and processed in the perinuclear nuage. We show that the Drosophila nuclear DEAD box protein UAP56, previously implicated in mRNA splicing and nuclear export, co-localizes with the cluster-assoc...
ORGANISM(S): Drosophila melanogaster 
2012-11-28 | E-GEOD-35637 | biostudies-arrayexpress
TranscriptomicsIdentification of WRKY22 targets under submergence in Arabidopsis (mRNA)
We analyzed global transcriptional changes in submerged Arabidopsis seedlings comparing control untreated seedlings. The same analysis were also applied on wrky22-ko2 seedling to identify WRKY22 targets under submergence. Time course experiments (1, 3, and 6 for Columbia and wrky22-ko2). Tissues f...
ORGANISM(S): Arabidopsis thaliana 
2013-07-03 | E-GEOD-40139 | biostudies-arrayexpress
UnknownDevelopment of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model.
Not available
S-EPMC6300396 | biostudies-literature
TranscriptomicsChromatin immunoprecipitation of Rok-myc and Hbs-myc in Bacillus subtilis
The Rok protein of Bacillus subtilis was identified as a negative regulator of competence development. Here we show that Rok binds to extended areas of the B. subtilis genome that are characterized by a high A+T content and are known or believed to have been acquired by horizontal gene transfer, e.g...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2011-01-01 | E-GEOD-23199 | biostudies-arrayexpress
GenomicsThe Histone H3 Lysine 9 Methyltransferases G9a and GLP Regulate Polycomb Repressive Complex 2-Mediated Gene Silencing [ChIP-Seq]
G9a/GLP and Polycomb Repressive Complex 2 (PRC2) are two major epigenetic silencing machineries, which in particular methylate histone H3 on lysines 9 and 27 (H3K9 and H3K27), respectively. Although evidence of a crosstalk between H3K9 and H3K27 methylations has started to emerge, their actual inter...
ORGANISM(S): Mus musculus 
2014-01-03 | E-GEOD-46536 | biostudies-arrayexpress
TranscriptomicsTranscriptional characterization of maize roots in response to urea and nitrate
Although urea is the most used nitrogen fertilizer worldwide, little is known on the capacity of crop plants to use urea per se as a nitrogen source for development and growth. To date, the molecular and physiological bases of its transport have been investigated only in a limited number of species....
ORGANISM(S): Zea mays 
2015-03-02 | E-GEOD-53102 | biostudies-arrayexpress
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