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Naa50 regulates tapetum degradation
TMT-labeled LC-MSMS was performed to identify and quantify proteins from three different TAP-purification pull-outs from Chaetomium thermophilum. The aim was to identify interaction partners and to study, whether the two tagged proteins (Naa50 and Naa15) are likely to interact, as they do in other o...
ORGANISM(S): Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) 
2022-10-14 | PXD035320 | Pride
Transcriptome profiling following knockdown of NAA50 in Arabidopsis
N-terminal acetylation of proteins is a key modification in eukaryotes; however, our understanding of its biological function in plants is limited. Naa50 is the catalytic subunit of the protein N-terminal acetyltransferase NatE complex. We previously showed that the absence of Naa50 led to sterility...
ORGANISM(S): Arabidopsis thaliana 
2022-05-31 | GSE175392 | GEO
Nα-terminal acetylation (NTA) ranges among the most abundant protein modifications in higher eukaryotes. Over 40 % of the human and plant proteome are co-translationally acetylated by a single Nα-acetyltransferase (Nat) termed NatA. The core NatA complex consists of the catalytic subunit NAA10 and t...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
We utilized RNA-seq to identify transcriptome changes following knockdown of the Arabidopsis N-terminal acetyltransferase NAA50. Transgenic Arabidopsis plants carrying a dexamethasone-inducible amiRNA were used to study the effects of NAA50 knockdown. Significant changes occurred in the transcriptom...
ORGANISM(S): Arabidopsis thaliana 
2020-02-20 | GSE145580 | GEO
N-terminal acetylation is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-terminal acetyltransferases. AtNAA50 interreact with the NatA complex (NAA10 and NAA15) to form the NatE complex. In this study, we investigated the Arabidopsis thaliana N-ter...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-07-15 | PXD017770 | Pride
Co-translational N-terminal (Nt-) acetylation of nascent polypeptides is catalyzed by N-terminal acetyltransferases (NATs). The very N-terminal amino acid sequence is the major factor determining whether or not a given protein is Nt-acetylated. In humans, six different NATs, denoted NatA-NatF, are i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-05-29 | PXD001437 | Pride
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