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ProteomicsMass spectrometry for SUMO site detection from 3×Flag-SUMO-T86R expressing cells
Anti-Flag immunoprecipitated 3×Flag-SUMO-T86R proteins on beads were washed once and suspended in 50 mM ammonium bicarbonate, followed by the addition of 1 µg trypsin/Lys-C mix and an overnight incubation at 37ºC. The digests were reduced, alkylated, diluted 10-fold with HBS (50 mM HEPES-NaOH pH7.5,...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-09-19 | PXD068511 | JPOST Repository
ProteomicsProteomic analysis of SovN-associated proteins immunopurified with an anti-FLAG antibody
Immunoprecipitated proteins on beads were washed once and resuspended in 50 mM ammonium bicarbonate, followed by the addition of 200 ng trypsin/Lys-C mix and an overnight incubation at 37ºC. The digests were reduced, alkylated, acidified, and desalted with GL-Tip SDB. Then, the eluates were evaporat...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-09-19 | PXD068509 | JPOST Repository
ProteomicsProteomic analysis of Panx- and Nxf2-associated proteins immunopurified with an anti-HA antibody
Immunoprecipitated proteins on beads were washed once and resuspended in 50 mM ammonium bicarbonate, followed by the addition of 200 ng trypsin/Lys-C mix (Promega) and an overnight incubation at 37ºC. The digests were reduced, alkylated, acidified, and desalted with GL-Tip SDB. Then, the eluates wer...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-09-19 | PXD068508 | JPOST Repository
ProteomicsProteomic analysis of PNUTS-associated proteins immunopurified with an anti-HA antibody
Immunoprecipitated proteins on beads were washed once and resuspended in 50 mM ammonium bicarbonate, followed by the addition of 200 ng trypsin/Lys-C mix and an overnight incubation at 37ºC. The digests were reduced, alkylated, acidified, and desalted with GL-Tip SDB. Then, the eluates were evaporat...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-09-19 | PXD068513 | JPOST Repository
ProteomicsMass spectrometry for SUMO site detection from 3×Flag-SUMO-T86K expressing cells
Anti-Flag immunoprecipitated 3×Flag-SUMO-T86K proteins on beads were washed once and suspended in 50 mM ammonium bicarbonate, followed by the addition of 1 µg Lys-C and an overnight incubation at 37ºC. The digests were reduced, alkylated, diluted 10-fold with HBS (50 mM HEPES-NaOH pH7.5, 150 mM NaCl...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-09-19 | PXD068512 | JPOST Repository
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