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TranscriptomicsIdentification of genes up-regulated by the overexpression of HSF1 in human HeLa cells
To analyze target genes of human heat shock transcription factor 1 (HSF1), we first generated two independent HeLa clones (RDT1 and RDT2) expressing an actively mutated hHSF1 (hHSF1?RDT), which lacks the regulatory domain that masks its activation domain and possesses a glutamic acid at amino acid 3...
ORGANISM(S): Homo sapiens 
2010-09-22 | E-GEOD-19797 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse cell culture treated with LPS to identify of inflammatory genes suppressed by heat shock
To clarify inflammatory genes whose expression is suppressed at high temperatures, we performed comprehensive analysis of gene expression by using a DNA microarray. Two independent primary cultures of mouse embryo fibroblasts (MEF1 and MEF2) were treated with LPS for 4 hours, or treated with LPS for...
ORGANISM(S): Mus musculus 
2010-01-19 | E-GEOD-16266 | biostudies-arrayexpress
ProteomicsTRRAP-interacting proteins during heat shock
Project description: HeLa cells and cells stably expressing hTRRAP-3xFlAG were subjected to mass spectrometry (MS) analysis. Briefly, Cells were treated or untreated with heat shock at 42°C for 30 min, and TRRAP was immunoprecipitated from these cell extracts with 60 ul anti-FLAG M2 affinity gels by...
ORGANISM(S): Homo Sapiens (human) 
2022-06-19 | PXD031822 | JPOST Repository
TranscriptomicsChromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and their denucleation [lens tissue]
Genome-wide approach to identify the cell-autonomous role of Brg1 in lens fiber cell terminal differentiation. To examine roles of Brg1 in mouse lens development, a dnBrg1 transgenic construct was expressed using the lens-specific alphaA-crystallin promoter in postmitotic lens fiber cells. Morpholog...
ORGANISM(S): Mus musculus 
2011-01-01 | E-GEOD-22322 | biostudies-arrayexpress
TranscriptomicsIdentification of genes regulated by HSF1 in control and heat-shocked cells
To examine the regulation of HSF1-mediated gene expression in control and heat shock conditions, we performed DNA microarray analysis. mRNA levels in control and heat-shocked MEF cells, which were infected with adenovirus expressing scramble RNA or HSF1 short hairpin RNA, were analyzed by DNA microa...
ORGANISM(S): Mus musculus 
2014-11-01 | E-GEOD-54060 | biostudies-arrayexpress
ProteomicsHSF1-interacting proteins in control and heat shock conditions
HeLa cells were treated or untreated with heat shock at 42°C for 5, 30, and 60 min, and were then fixed in medium containing 1% formaldehyde at 37°C for 30 min. Chromatin fraction was sonicated with the Branson Sonifier 450 (BRANSON Ultrasonics) into fragmented DNA around 200-300 bp. HSF1 bound to g...
ORGANISM(S): Homo Sapiens (human) 
2022-06-19 | PXD031821 | JPOST Repository
TranscriptomicsHSF4 microarray gene expression analysis in the newborn mouse lens.
Differential expression of HSF4 in null newborn mouse and wildtype lenses was examined to identify putative downstream targets of HSF4. To examine roles of Brg1 in mouse lens development, a dnBrg1 transgenic construct was expressed using the lens-specific aA-crystallin promoter in postmitotic lens f...
ORGANISM(S): Mus musculus 
2010-12-01 | E-GEOD-22362 | biostudies-arrayexpress
GenomicsGenome wide trimethyl-H3K27 distibution in macrophages from wild-type and Jmjd3-/- macrophages.
Jmjd3 is trimethyl H3K27 specific demethylase required for M2 macrophage polarization. Genomic fragments obtained from wild-type and Jmjd3-/- mouse macrophages were immunoprecipitated with anti H3K27me3 Ab, and deep sequencing was performed. wild-type and Jmjd3-/- macrophages
ORGANISM(S): Mus musculus 
2010-08-22 | E-GEOD-23297 | biostudies-arrayexpress
TranscriptomicsGenome-wide DNA methylation profiling in cultured eutopic and ectopic endometrial stromal cells (expression)
Transcriptome profiles were investigated in isolated endometrial stromal cells (ESCs) from eutopic and ectopic endometrium. The profiles were quite different between eutopic ESC and ectopic ESC, whereas no clear dfference was recognized between eutopic ESC with and without endometriosis. Total RNA f...
ORGANISM(S): Homo sapiens 
2013-09-07 | E-GEOD-47360 | biostudies-arrayexpress
TranscriptomicsGenome-wide DNA methylation profiling in cultured eutopic and ectopic endometrial stromal cells (methylation)
Profiles of genome-wide DNA methylation were investigated in isolated endometrial stromal cells from eutopic and ectopic endometrium. DNA methylation profiles were quite different between eutopic ESC and ectopic ESC, whereas no clear dfference were recognized between eutpic ESC with and without endo...
ORGANISM(S): Homo sapiens 
2013-09-07 | E-GEOD-47359 | biostudies-arrayexpress
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