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GenomicsGenome-wide identification of CCA1-bound genes in Arabidopsis by ChIP-Seq
LHY and CCA1 encode single MYB transcription factors, involved in circadian clock. However, direct target genes of LHY and CCA1 in a genomic scale were largely unknown. To reveal bound genes by CCA1, chimeric protein CCA1-FLAG was expressed under CCA1 promoter in cca1 lhy (CCA1pro:CCA1-FLAG/ cca1 lh...
ORGANISM(S): Arabidopsis thaliana 
2016-03-11 | E-GEOD-67903 | biostudies-arrayexpress
TranscriptomicsGene expression in the epidermis including guard cells of phot1 phot2 and pGC1::SOC1-GFP/phot1 phot2
Stomata pores are surrounded by a pair of guard cells in the epidermis in plants. Stomata apature is controlled by environmental factors. Stomata opening is also enhanced by introduction of SOC1-GFP driven by GC1 promoter in phot1 phot2 double mutants (pGC1::SOC1-GFP/ phot1 phot2). Epidermis includi...
ORGANISM(S): Arabidopsis thaliana 
2015-02-04 | E-GEOD-60183 | biostudies-arrayexpress
ProteomicsTOC1 and PRR5 as regulators of temperature compensation in the Arabidopsis circadian clock (12˚C vs 28˚C)
Ambient temperature potentially affects all biological systems. However, the period length of circadian clocks is close to 24 h over a broad range of physiological temperatures, known as temperature compensation. The mechanism underpinning the temperature compensation remains largely unknown. Here, ...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2024-10-04 | PXD044813 | JPOST Repository
TranscriptomicsThe effect of over-expression of PRR5-VP in Arabidopsis seedlings
PRR5 transcription factor acts in the circadian clock system. To elucidate regulated genes by PRR5, Chimeric protein PRR5-VP, which activates direct target genes of PRR5, was over-expressed in Col-0. Microarray analsysis was performed using these plants with Affymetrix ATH1 genechip. PRR5-VP express...
ORGANISM(S): Arabidopsis thaliana 
2012-09-20 | E-GEOD-36360 | biostudies-arrayexpress
GenomicsGenome-wide maps of PRR5 binding loci in Arabidopsis thaliana
PRR5 transcription factor acts in the circadian clock system. To elucidate bound genes by PRR5, Chimeric protein FLAG-PRR5-GFP, was expressed under PRR5 promoter in Col-0 (PRR5pro:FLAG-PRR5-GFP/prr5). ChIP was performed using anti-GFP antibody (ab290;Abcam), which was bound to Dynabeads Protein G (1...
ORGANISM(S): Arabidopsis thaliana 
2012-09-20 | E-GEOD-36361 | biostudies-arrayexpress
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