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UnknownLong-Term Implantable Cardioverter Defibrillator Lead Dysfunction After Left Ventricular Assist Device Implantation.
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S-EPMC12595381 | biostudies-literature
ProteomicsTMTpro-18plex analysis of siSTK38 or siLuc HeLa cells treated with EBSS or LLOMe
HeLa cells were transfected with STK38 siRNA or Luc siRNA and then treated with EBSS or LLOMe in three biological replicates. The cells were lysed in 500 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (330...
ORGANISM(S): Homo Sapiens (human) 
2023-11-21 | PXD039312 | JPOST Repository
ProteomicsTMT-based proteomic analysis of Atg5-/- mouse brain
The brains of neonatal mice (Atg5+/+ in triplicate, Atg5-/- in quadruplicate, and Atg5-/-;NSE-Atg5 in triplicate) were lysed in 500 µL of 6 M guanidine-HCl containing 100 mM Tris-HCl, pH8.0, and 2 mM DTT. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 g fo...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-01 | PXD049220 | JPOST Repository
ProteomicsProximity proteomics of T-tubules using miniTurbo-fused LUBEL
To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an LUBEL construct fused with miniTurbo (mnTb). We expressed either LUBEL-mnTb or mnTb alone in muscles, and larvae were reared in the presence o...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062634 | JPOST Repository
ProteomicsCo-IP and MS analysis of LacZ or 3xHA:LUBEL-N
HA-LUBEL 1-872 or LacZ (control) was expressed under the control of Mef2-GAL4, and the progenies were raised at 25°C. The 3IL carcass fillet lysates were subjected to anti-HA immunoprecipitation. To identify potential binding partners, we performed immunoprecipitation-mass spectrometry (IP-MS) of LU...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062558 | JPOST Repository
ProteomicsProximity proteomics of T-tubules
To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an Amph construct fused with miniTurbo (mnTb). We expressed either Amph-mnTb or mnTb alone in muscles, and larvae were reared in the presence or ...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062543 | JPOST Repository
ProteomicsProteomics analysis of proteins co-immunoprecipitated with MIDN-Flag in PC-12 cells
We have previously identified midnolin (MIDN) as a genetic risk factor for Parkinson's disease. However, the molecular entity of MIDN that regulates neuronal phenotypes remains unclear. To investigate the molecular function of MIDN, we examined proteins associated with MIDN in PC-12 cells. We perfor...
ORGANISM(S): Rattus Rattus (black Rat) 
2024-09-13 | PXD053265 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing TFEB-mNeonGreen
Cells stably expressing TFEB-mNeonGreen proteins were grown in a 10 cm dish and treated with or without LLOMe for 1 h or 3 h, then cross-linked with 0.1% formaldehyde for 10 min at room temperature. After cross-linking was quenched with 100 mM glycine for 4 min at room temperature, cells were washed...
ORGANISM(S): Homo Sapiens (human) 
2025-08-17 | PXD043288 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing TFEB-mNeonGreen treated with MK6-83
Cells stably expressing TFEB-mNeonGreen proteins were grown in a 10 cm dish and treated with or without MK6-83 for 3 h, then cross-linked with 0.1% formaldehyde for 10 min at room temperature. After cross-linking was quenched with 100 mM glycine for 4 min at room temperature, cells were washed with ...
ORGANISM(S): Homo Sapiens (human) 
2025-08-17 | PXD053327 | JPOST Repository
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing HKDC1-mNeonGreen
Cells stably expressing full-length HKDC1-mNeonGreen or △N20 HKDC1-mNeonGreen proteins were grown in a 10-cm dish and were treated with or without antimycin/oligomycin for 3h, and then cross-linked with 0.1% formaldehyde for 10min at room temperature. After cross-linking was quenched with 100mM glyc...
ORGANISM(S): Homo Sapiens (human) 
2024-01-21 | PXD040970 | JPOST Repository
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