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E11 murine kidney podocyte cell line was purchased from Cell Lines Service. Cells were cultured in RPMI medium (Wako) supplemented with 10% fetal bovine serum (Sigma) at 33°C and induced to differentiate by culture at 37°C for two weeks and 5% CO2. Cdkal1 knockout in the E11 cell line was performed...
ORGANISM(S): Mus Musculus (mouse) 
PDZD8-TurboID knocked-in HeLa cells were plated into a 15 cm dish and cultured two overnight. The cells were treated with Biotin at the final concentration of 50 μM, and incubated for 6 hours. The cells were washed twice with ice-cold Hepes-saline and lysed in 6 M guanidine-HCl containing 100 mM HEP...
ORGANISM(S): Homo Sapiens (human) 
Pdzd8-3xHA knock-in mice and control littermates at postnatal 10 days were put to sleep using medetomidine hydrochloride, midazolam and butorphanol. Pups were then put on the ice for 5 minutes and exsanguinated by terminal intracardial perfusion with ice-cold 2% paraformaldehyde in PBS. The neocorte...
ORGANISM(S): Mus Musculus (mouse) 
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