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ProteomicsTMT-based phosphoproteomic analysis of cardiac proto-myofibroblasts after Bcat1 knockdown
Proto-myofibroblasts were treated with siRNA, washed with ice-cold HEPES-saline (20 mM HEPES-NaOH [pH 7.5], 137 mM NaCl), detached by Accutase (Nacalai Tesque), and lysed in guanidine-TCEP buffer (8 M guanidine-HCl, 100 mM HEPES-NaOH [pH 7.5], 10 mM TCEP, 40 mM CCA). After heating and sonication, 20...
ORGANISM(S): Mus Musculus (mouse) 
2026-07-08 | PXD075668 | JPOST Repository
ProteomicsTMT-based quantitative proteomic analysis of cardiac proto-myofibroblasts after Bcat1 knockdown
Proto-myofibroblasts were treated with siRNA, washed with ice-cold HEPES-saline (20 mM HEPES-NaOH [pH 7.5], 137 mM NaCl), detached by Accutase (Nacalai Tesque), and lysed in guanidine-TCEP buffer (8 M guanidine-HCl, 100 mM HEPES-NaOH [pH 7.5], 10 mM TCEP, 40 mM CCA). After heating and sonication, 20...
ORGANISM(S): Mus Musculus (mouse) 
2026-07-08 | PXD075667 | JPOST Repository
ProteomicsNakaya_VGLL3-FLAG_IP-MS_QEplus
Cardiac myofibroblasts retrovirally transduced with FLAG-VGLL3 or control vector were fixed with 0.1% PFA and lysed in a HEPES-RIPA buffer (20 mM HEPES-NaOH [pH 7.5]/1 mM EGTA/1 mM MgCl2/150 mM NaCl/0.25% sodium deoxycholate/0.05% SDS/1% NP-40) containing protease inhibitors (1:100), phosphatase inh...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039091 | JPOST Repository
ProteomicsImmunnoprecipitation followed by PRM to detect endogenous interaction between VGLL3 and DDX5
For immunoprecipitation to detect the endogenous interaction between VGLL3 and DDX5, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhibi...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039093 | JPOST Repository
ProteomicsImmunoprecipitation followed by PRM to detect endogenous interaction between VGLL3 and EWSR1
For immunoprecipitation to detect the endogenous interaction between VGLL3 and EWSR1, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhib...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039092 | JPOST Repository
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