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RNAP II is frequently paused near gene promoters in mammals and its transition to productive elongation requires active recruitment of P-TEFb, a cyclin-dependent kinase for RNAP II and other key transcription elongation factors. A fraction of P-TEFb is sequestered in an inhibitory complex containing...
ORGANISM(S): Mus musculus 
Co-translational binding of importins to nascent proteins
We combined the nuclear run-on (NRO) assay which labels and captures nascent transcripts with high throughput DNA sequencing to examine transcriptional activity in Saccharomyces cerevisiae. Examination of nascent transcripts and steady-state transcripts in exponentially growing and heat-shock treate...
ORGANISM(S): Saccharomyces cerevisiae 
Nascent RNA antagonises the interaction of a set of regulatory proteins with chromatin
we sought to determine whether dmAGO1 and Ltn1 share common ubiquitinated nascent peptide targets. For this purpose, we performed the UBA bead-based immunoprecipitation followed by mass spec experiments to identify the ubiquitinated nascent peptide targets of dmAGO1 and Ltn1, respectively. In this a...
ORGANISM(S): Drosophila Melanogaster 
2022-02-18 | PXD031749 |
Nascent allohexaploid wheat may represent the initial genetic state for the evolution and domestication of common wheat, which arose by combining the AB genomes of tetraploid Triticum turgidum with the D genome from Aegilops tauschii and out-competed its parents in growth vigor and adaptability. To ...
ORGANISM(S): Triticum dicoccon 
Long read SMRT cDNA sequencing of nascent RNA from exponentially growing S. cerevisiae and S. pombe cells was employed to obtain transcription elongation and splicing information from single transcripts. Nascent RNA was prepared from the yeast chromatin fraction (Carrillo Oesterreich, Preibisch, Neu...
ORGANISM(S): Schizosaccharomyces pombe 
Alcoholic liver disease (ALD) poses a significant global health challenge, and the global proteome will change dynamically and dramatically for the response to alcohol stress. However, due to the lack of method for labeling and identification of tissue-specific nascent proteome in living mouse, the ...
ORGANISM(S): Homo Sapiens Mus Musculus 
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