This dataset consists of 44 raw MS files, comprising 27 DIA (SWATH) and 15 DDA runs on a TripleTOF 5600 and of two raw mass spectrometry files acquired on a Q Exactive. The composition of the dataset is described in the manuscript by Tsou et al., titled: "DIA-Umpire: comprehensive computational fra...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606)
This dataset consists of 44 raw MS files, comprising 27 DIA (SWATH) and 15 DDA runs on a TripleTOF 5600 and of two raw mass spectrometry files acquired on a Q Exactive. The composition of the dataset is described in the manuscript by Tsou et al., titled: "DIA-Umpire: comprehensive computational fra...
ORGANISM(S): Homo sapiens (Human) Escherichia coli
Affinity purification-mass spectrometry (AP-MS) datasets of eleven human histone deacetylases (HDACs). Affinity-tagged (GFP) HDACs were expressed in a human CEM-T cell line. For label-free analysis, proteins were digested by trypsin in-solution and peptides separated across a linear 180 min gradient...
We used TMT-MS to compare protein abundance in conditioned media and cell lysates collected from control, LMAN1- or SURF4-deficient HuH7 cells to identify proteins that depend on these cargo receptors for secretion
This dataset includes BioID for SEC23A and SEC23B. The paper describes the functional redundancy of these two gene paralogs in the context of human disease.
The project aims to evaluate the use of a linear ion trap to increase the sensitivity of multiplexed single-cell proteomics. Using diluted mouse peptide samples from C10 and Raw, we compared the proteome coverages, data missingness, and quantification performance between linear ion trap and Orbitrap...