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TranscriptomicsNET-seq of S. cerevisiae cells after knocking-out genes encoding various mRNA decay factors
NET-seq was performed on yeast strains carrying deletions of mRNA decay factors (plus a control) in order to assess the effect of their deletion on RNA polymerase II occupancy at genetic loci. This is in pursuit of understanding the process of mRNA buffering which links mRNA synthesis and decay.
ORGANISM(S): Saccharomyces cerevisiae 
2020-02-08 | E-MTAB-8749 | biostudies-arrayexpress
GenomicsRNA Polymerase II transcription independent of TBP [NET-seq]
RNA Polymerase II transcription independent of TBP [NET-seq]
PRJNA851782 | ENA
TranscriptomicsNative elongating transcript sequencing (NET-seq) of wild type Saccharomyces cerevisiae and of DST1, RCO1, SET1, SET2, EAF3 deletion strains
We present an approach (native elongating transcript sequencing, NET-seq), based on deep sequencing of 3M-bM-^@M-^Y ends of nascent transcripts associated with RNA polymerase, to monitor transcription at nucleotide resolution. Application of NET-seq in Saccharomyces cerevisiae reveals that while pro...
ORGANISM(S): Saccharomyces cerevisiae 
2011-01-19 | E-GEOD-25107 | biostudies-arrayexpress
TranscriptomicsNative Elongating Transcript sequencing (NET-seq) in wild-type and three members of the CAF-I complex.
We performed a fluorescent reporter based screen to identify factors determining transcriptional directionality from bidirectional promoters that give rise to a coding and a non-coding transcript. Promoters like these are most frequent in many organisms and non-coding transcription from this origin ...
ORGANISM(S): Saccharomyces cerevisiae 
2014-06-01 | E-GEOD-55982 | biostudies-arrayexpress
TranscriptomicsRNA-Seq Profiling of a Defective Seed Coat Mutation in Glycine max Reveals Differential Expression of Proline Rich and Other Cell Wall Protein Transcripts
The plant cell wall performs a number of essential functions including providing shape to many different cell types and serving as a defense against potential pathogens. The net pattern mutation creates breaks in the seed coat of soybean (Glycine max) because of ruptured cell walls. Using RNA-Seq, w...
ORGANISM(S): Glycine max 
2014-05-16 | E-GEOD-54903 | biostudies-arrayexpress
GenomicsCoordination of widespread transcription at enhancers and target genes through BRD4 (His-NET-Seq)
Coordination of widespread transcription at enhancers and target genes through BRD4 (His-NET-Seq)
PRJNA886066 | ENA
GenomicsTEF-seq/NET-seq applied to factors Rpb3, Paf1, Spt6, Spt16, Cet1, Pcf11, Ssu72, Set2
TEF-seq/NET-seq applied to factors Rpb3, Paf1, Spt6, Spt16, Cet1, Pcf11, Ssu72, Set2
PRJEB42483 | ENA
GenomicsThe effect of metal cations on the capture of Pol II-derived nascent transcripts in NET-seq experiments
The effect of metal cations on the capture of Pol II-derived nascent transcripts in NET-seq experiments
PRJNA1109721 | ENA
GenomicsTesting the usage of divalent metal cations and nucleases during NET-seq for Pol I
Testing the usage of divalent metal cations and nucleases during NET-seq for Pol I
PRJNA893665 | ENA
GenomicsNET-seq of S. cerevisiae cells after knocking-out genes encoding various mRNA decay factors
NET-seq of S. cerevisiae cells after knocking-out genes encoding various mRNA decay factors
PRJEB36492 | ENA
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