Irgb6-deficient MEFs stably expressing Spot-tagged Irgb6 were infected with or without T. gondii ME49 for 4 h and lysed in guanidine buffer (6 M guanidine-HCl, 100 mM Tris-HCl, pH 8.0, and 2 mM DTT). The lysates were diluted 8-fold with RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl, 1 mM EGTA, ...
ORGANISM(S): Mus Musculus (mouse)
