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TranscriptomicsIdentification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algor
We present a microarray experiment with the same tet-inducible system but with multiple time points within 24 hr to capture early responses to Oct4 suppression. These data were analyzed together with published genome-wide ChIP data. We found that most tentative target genes (TTG) of Oct4 in ES cells...
ORGANISM(S): Mus musculus 
2008-04-24 | E-GEOD-8617 | biostudies-arrayexpress
TranscriptomicsExpression data from inducible ES stable cell line
In order to identify the effects of the induction of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the inducible not-tagged cell line. Transcriptome analysis of the inducible transgenic mouse ES cell line. The E13 inducible cell l...
ORGANISM(S): Mus musculus 
2012-12-13 | E-GEOD-31166 | biostudies-arrayexpress
TranscriptomicsDissecting Oct3/4-regulated gene networks in embryonic stem cells by expression
POU transcription factor Pou5f1 (Oct3/4) is required to maintain ES cells in an undifferentiated state. Here we show that global expression profiling of Oct3/4-manipulated ES cells delineates the downstream target genes of Oct3/4. Combined with data from genome-wide chromatin-immunoprecipitation ass...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-5936 | biostudies-arrayexpress
TranscriptomicsKlf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells
To characterize Klf4 downstream genes, we performed microarray analyses of the ES cells knocked down by siRNA of Klf4 gene. Keywords: individual genetic characteristic design ES cells grow without feeders on gelatin coated in complete ES medium. Cells were incubated at 37 0C; 5% CO2. ES cells were t...
ORGANISM(S): Mus musculus 
2010-06-10 | E-GEOD-5930 | biostudies-arrayexpress
TranscriptomicsGene expression profile after Dax1 deletion in ES cells
To investigate the function of Dax1 in mouse ES cells, whole genome microarray expression profiling was employed to identify genes that are potentially affected by the deletion of Dax1. An inducible knockout ES cell line of Dax1 with the Cre-loxP system was generated and gene expression was measured...
ORGANISM(S): Mus musculus 
2015-05-13 | E-GEOD-61152 | biostudies-arrayexpress
TranscriptomicsZscan10 is dispensable for maintenance of pluripotency in mouse embryonic stem cells
Zinc finger and SCAN domain-containing 10 (Zscan10, also known as Zfp206) encodes a transcription factor that has been reported to be involved in the maintenance of pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Zscan10 using the Cre-loxP system an...
ORGANISM(S): Mus musculus 
2015-12-20 | E-GEOD-76143 | biostudies-arrayexpress
ProteomicsMEAF6 is essential for proliferation and plays a role in the assembly of KAT7 complexes
MEAF6 is essential for proliferation and plays a role in the assembly of KAT7 complexes.
ORGANISM(S): Mus Musculus (mouse) 
2020-09-23 | PXD020422 | JPOST Repository
TranscriptomicsConditional knockout of Sox2 in embryonic and trophoblast stem cells.
To compare the transcriptional networks governed by Sox2 in embryonic stem (ES) cells and trophoblast stem (TS) cells, we performed whole-genome expression analysis after tetracycline (Tet)-induced knockout of Sox2 in each cell type. A Tet-inducible Sox2 knockout ES cell line 2TS22C or its derivativ...
ORGANISM(S): Mus musculus 
2013-10-17 | E-GEOD-28451 | biostudies-arrayexpress
TranscriptomicsInduction of trophoblast stem cells from embryonic stem cells by forced repression of Oct3/4.
To characterize the transdifferentiation of embryonic stem (ES) cells into trophoblast stem (TS) cells triggered by forced repression of Oct3/4, we performed whole-genome expression analysis after tetracycline (Tet)-induced knockout of Oct3/4. A Tet-inducible Oct3/4 knockout ES cell line ZHBTc4 was ...
ORGANISM(S): Mus musculus 
2013-10-17 | E-GEOD-28452 | biostudies-arrayexpress
GenomicsDynamics of Sox2 occupancy during the differentiation of embryonic stem cells into trophoblast stem cells.
To understand the mechanism underlying the versatility in transcriptional regulation by Sox2, we compared genome-wide binding sites of Sox2 in embryonic stem (ES) cells and trophoblast stem (TS) cells by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). A tetracycline-...
ORGANISM(S): Mus musculus 
2013-10-17 | E-GEOD-28453 | biostudies-arrayexpress
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