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TranscriptomicsGenome-wide profiling of gene expression of normal and Tbx20 knockout adult mouse whole heart
Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we generated a conditional knockout for this gene, specifically in cardiomyocytes. We profiled gene expression levels using RNA-seq in both normal and knockout adult mouse hearts to ident...
ORGANISM(S): Mus musculus 
2012-02-10 | E-GEOD-30943 | biostudies-arrayexpress
GenomicsGenome-wide mapping of Tbx20 (GFP-tagged) in adult mouse whole heart
Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we sought to identify regulatory regions, gene targets and pathways regulated by this TF. To this end, we used chromatin immunoprecipitation (ChIP) of Tbx20 fused to GFP to locate binding...
ORGANISM(S): Mus musculus 
2011-11-21 | E-GEOD-29636 | biostudies-arrayexpress
TranscriptomicsPromoter capture Hi-C of human SGBS (Simpson-Golabi-Behmel syndrome preadipocyte) cells and iPSC-derived hypothalamic cells
To identify distal chromatin contacts between promoters and their putative regulatory elements in SGBS preadipocytes and hypothalamic arcuate-like neurons derived from iPSC, we performed Hi-C with a capture step to enrich the library for contacts involving promoters. Hi-C with a capture step was per...
ORGANISM(S): Homo sapiens 
2021-03-20 | E-MTAB-10200 | biostudies-arrayexpress
TranscriptomicsGene expression analysis of the effect of Irx3 knockout in mouse hypothalamus compared with wild-type (in high-fat diet)
To establish the roles of Irx3 in molecular and physiological brain phenotypes associated with obesity, we performed transcriptomic analysis (RNA-seq) on hypothalami from Irx3 CRISPR/Cas9 knockout mice and wild-type littermates.
ORGANISM(S): Mus musculus 
2021-03-14 | E-MTAB-10186 | biostudies-arrayexpress
TranscriptomicsSingle cell sequencing of wild-type and mutant iPSC-derived hypothalamic arcuate-like neurons cells (deletion of hg19:chr16:53,796,691-53,832,771 / hsFTOΔ36)
In this experiment, we deleted 36,100 bp of the first intron of the human FTO gene to test whether this region contains transcriptional enhancers for RPGRIP1L, FTO, IRX3, IRX5, and IRX6 genes. The deletion was performed in iPSC and we differentiated wild-type and hsFTOΔ36 cells into hypothalamic pro...
ORGANISM(S): Homo sapiens 
2021-12-01 | E-MTAB-10201 | biostudies-arrayexpress
TranscriptomicsObesity-associated variants within FTO form long-range functional connections with IRX3
Genome-wide association studies in diverse populations have reproducibly associated variants within introns of FTO with increased risk for obesity and type-2 diabetes.While the molecular mechanisms linking these noncoding variants with obesity are not immediately obvious, subsequent studies in mice ...
ORGANISM(S): Danio rerio 
2014-02-24 | E-GEOD-52830 | biostudies-arrayexpress
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