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Non-AUG translation initiation is crucial for regulating translation, which can generate N-terminally extended protein isoforms with diverse biological functions. However, a comprehensive view of non-AUG translation initiation events with N-terminal extensions (NTEs) across monocots and dicots remai...
ORGANISM(S): Glycine max Zea mays (Maize) Oryza sativa (Rice) 
2026-04-27 | PXD058528 | Pride
Translation initiation site profiling reveals widespread synthesis of non-AUG-initiated protein isoforms in yeast
Techniques for systematically monitoring protein translation have lagged far behind methods for measuring mRNA levels. Here we present a ribosome profiling strategy, based on deep sequencing of ribosome protected mRNA fragments, that enables genome-wide investigation of translation with sub-codon re...
ORGANISM(S): Saccharomyces cerevisiae 
Negative elongation factor (NELF), a four-subunit protein complex in metazoan, plays an important role in regulating promoter-proximal pausing of RNA polymerase II (RNAPII). Genetic studies demonstrate that the B subunit of mouse NELF (NELF-B) is critical for embryonic development and homeostasis i...
ORGANISM(S): Mus musculus 
Ribosome queuing enables non-AUG translation to be resistant to multiple protein synthesis inhibitors
The goal of this study is to evaluate the competition between eIF5 and 5MP in utilizing non-AUG translation initiation sites
ORGANISM(S): Homo sapiens 
2017-08-18 | GSE102786 | GEO
Genomic analyses in budding yeast have helped to define the foundational principles of eukaryotic gene expression, but have systematically excluded specific classes of potential coding regions, including those with non-AUG start codons. Without methods to define coding regions empirically, the preva...
ORGANISM(S): Saccharomyces cerevisiae 
2020-07-24 | GSE150375 | GEO
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