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ProteomicsHPLC-MS/MS analysis of the S-monooxygenated S-Trp (O=S-Trp) bearing peptide in heme reconstituted EcKatG S-Trp105
A sulfur-containing analog of tryptophan, thiotryptophan (3-benzothienyl-L-alanine, S-Trp), was genetically incorporated into EcKatG to replace the MYW-forming tryptophan, W105. The heme free apo-protein was purified, after heme-reconstitution and peroxide treatment, the S-Trp-bearing peptide was is...
ORGANISM(S): Escherichia coli 
2026-05-13 | PXD078069 | Pride
Catalase-peroxidase Non-canical amino acid Katg 
ProteomicsHPLC-MS/MS analysis of the S-monooxygenated S-Trp (O=S-Trp) bearing peptide in EcKatG S-Trp105
A sulfur-containing analog of tryptophan, thiotryptophan (3-benzothienyl-L-alanine, S-Trp), was genetically incorporated into EcKatG to replace the MYW-forming tryptophan, W105. The S-Trp-bearing peptide was isolated using trypsin digestion followed by HPLC separation. Tandem mass spectrometry (MS/M...
ORGANISM(S): Escherichia coli 
2026-05-13 | PXD078006 | Pride
Catalase-peroxidase Non-canical amino acid Katg 
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