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TranscriptomicsComparison of the DNA methylation of bovine blastocysts and bovine sperm
DNA from expanded bovine blastocysts and bovine sperm were extracted then subjected to methylation-sensitive enzymatic digestion and LM-PCR enrichment before being hybridized onto a microarray. Two-condition experiment, bovine blastocysts (pools of 10) vs bovine sperm. Four biological replicates of ...
ORGANISM(S): Bos taurus 
2014-09-09 | E-GEOD-57709 | biostudies-arrayexpress
TranscriptomicsExposure of bovine oocytes and embryos to elevated non-esterified fatty acid concentrations: integration of epigenetic and transcriptomic signatures in resultant blastocysts [DNA methylation]
Metabolic stress associated with negative energy balance in high producing dairy cattle and obesity in women is a risk factor for decreased fertility. Non-esterified fatty acids (NEFA) are involved in this pathogenesis as they jeopardize oocyte and embryo development. Growing evidence indicates that...
ORGANISM(S): Bos taurus 
2016-06-28 | E-GEOD-83766 | biostudies-arrayexpress
TranscriptomicsExposure of bovine oocytes and embryos to elevated non-esterified fatty acid concentrations: integration of epigenetic and transcriptomic signatures in resultant blastocysts [gene expression]
Metabolic stress associated with negative energy balance in high producing dairy cattle and obesity in women is a risk factor for decreased fertility. Non-esterified fatty acids (NEFA) are involved in this pathogenesis as they jeopardize oocyte and embryo development. Growing evidence indicates that...
ORGANISM(S): Bos taurus 
2016-06-28 | E-GEOD-83767 | biostudies-arrayexpress
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