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ProteomicsHigh-Throughput Proteomics Sample Preparation Using 96-Channel Pipettor and Magnetic Pin Device
The advent of high-throughput proteomics has created an urgent need for parallel increases in sample processing productivity. However, workflows—particularly those involving PTM profiling—remain highly complex and consist of multiple steps that demand exceptional reproducibility. Many laboratories a...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2026-02-17 | PXD069607 | Pride
Oasis hlb Proteomics Sp3 Phosphoproteomics Pac High-throughput samples preparation 
MetabolomicsGNPS Optimisation SPE Oasis HLB Prime - EMP500 fecal sample
GNPS Optimisation SPE Oasis HLB Prime (Hydrophilic-Lipophilic-Balance, 30 mg with particle sizes of 30 um) using an EMP500 fecal sample. LC-MS/MS analysis of the different washing/elution steps on the Q-Exactive, using a C18 polar column. https://gnps.ucsd.edu/ProteoSAFe/status.jsp?task=2d2bb492398...
ORGANISM(S): None 
2019-02-03 | MSV000083395 | GNPS
UnknownFacile purification and click labeling with 2-[18F]fluoroethyl azide using solid phase extraction cartridges.
Not available
S-EPMC4792033 | biostudies-literature
ProteomicsLiver proteomes from WT, Rcrin knockout, AAV-Ctrl and AAV-Rpl8 mice
The liver tissues were disrupted by the ultrasonic processor on ice in lysis buffer. After centrifugation, the extracted proteins were reduced with 10 mM DTT for 2 h at room temperature followed by alkylation with 20 mM iodoacetamide for 30 min in the dark. The protein solution was diluted 1:5 with ...
ORGANISM(S): Mus Musculus 
2024-03-28 | PXD051039 |
ProteomicsTargeted lipidomic analysis of PEX and POAG AH samples
Targeted lipidomic analysis of AH samples was carried out using UPLC-MS/MS. Samples underwent solid-phase extraction (SPE) using Oasis PRIME HLB cartridges (60 mg, 3 cc, #186008056, Waters, Eschborn, Germany) on a VacElut manifold (Agilent, Santa Clara, CA, USA).
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-10-24 | MSV000099582 | MassIVE
ProteomicsEGFR inhibition triggers an adaptive response by co-opting antiviral signaling pathways in lung cancer
HCC827 cells were treated with 0.1 microM erlotinib for 0, 2, 6, and 24 hours. Cell lysates were immunoprecipitated with TBK1 antibody. Antibody enriched protein samples were run on SDS-PAGE gels and submitted to UT Southwestern Proteomics Core Facility for Mass Spectrometry. Protein gel pieces were...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-03 | MSV000084654 | MassIVE
Proteomicspropionate bioconversion to methane
methane yield was promoted during propionate anaerobic digestion process.The total proteins of three samples were extracted, and the concentration and quality of proteins were determined by BCA Protein Assay Kit and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. ...
ORGANISM(S): Prokaryotic Environmental Samples 
2022-03-03 | PXD032020 |
ProteomicsAlcoholic hepatitis plasma degradome
It was hypothesized that the severe inflammatory liver injury caused by alcoholic hepatitis (AH) would yield a unique peptidome profile in human patient plasma, and degraded fragments (degradome) of hepatic and extracelluar proteomes would change between AH patient groups defined by MELD scores. Fo...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-27 | MSV000093513 | MassIVE
ProteomicsMultivalent coiled-coil interactions enable full-scale centrosome assembly and strength
Cross-linked samples consist of monomeric SPD-5 which were incubated with either kinase dead or constitutively active PLK-1 plus ATP-MgCl2 in a 150mM KCl, 25mM HEPES, pH7.4 buffer for 2 hours at room temperature. Samples were then cross-linked using 8mM DMTMM for 45min and quenched with 50 mM ammoni...
ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239) 
2024-01-05 | MSV000093775 | MassIVE
UnknownDevelopment and Validation of a UHPLC-ESI-MS/MS Method for Quantification of Oleandrin and Other Cardiac Glycosides and Evaluation of Their Levels in Herbs and Spices from the Belgian Market.
Not available
S-EPMC7232191 | biostudies-literature
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