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Lipids are being increasingly used as biodiesel feedstock, and several saturated wax esters from Euglena gracilis are candidates for outdoor bulk production. Wax ester fermentation in Euglena is strongly increased by hypoxia, but key events underlying the metabolic shift toward wax ester biosynthesi...
2014-09-04 | MTBLS113 | MetaboLights
Genome-wide scanning of gene expression by microarray techniques was successfully performed on RNA extracted from a sterilized soil inoculated with Pseudomonas putida KT2440/pSL1, which contains a chloroaromatic degrading plasmid, in the presence or absence of 3-chlorobenzoic acid (3CB). The genes s...
ORGANISM(S): Pseudomonas putida KT2440 
To analyze the gene expression of non-bacterial bladdder inflammation, mouse cyclophosphamide(CYP)-induced model of cystitis was adapted. Forty-eight hour after CYP or vehicle treatment, total RNA was extracted from CYP-treated group (CYP150) and control group (CYP0). We used microarrays to detail t...
ORGANISM(S): Mus musculus 
Transcriptomic analysis of the cells grown on biphenyl in the sterilized soil and those grown on biphenyl to mid-exponential phase in a liquid medium 4x dual channel slides.
ORGANISM(S): Rhodococcus jostii RHA1 
Transcroptomic analysis of the cells grown on pyruvate in the sterilized soil and those grown on pyruvate to mid-exponenyial phase in a liquid medium 4x dual channel slides.
ORGANISM(S): Rhodococcus jostii RHA1 
Cells stably expressing full-length HKDC1-mNeonGreen or △N20 HKDC1-mNeonGreen proteins were grown in a 10-cm dish and were treated with or without antimycin/oligomycin for 3h, and then cross-linked with 0.1% formaldehyde for 10min at room temperature. After cross-linking was quenched with 100mM glyc...
ORGANISM(S): Homo Sapiens (human) 
Data analyzed proteins extracted from lung tissue samples from OA-induced acute lung injury model rats. Samples were collected at 0 (pretreatment), 3, 6, 24, 48, 96 h after OA administration. Whole tissue extracts were prepared from three individuals and analyzed using the DIA-MS method.
ORGANISM(S): Cellular Organisms 
In recent years, deep learning-based in silico spectral libraries have gained increasing attention. Several data-independent acquisition (DIA) software tools have integrated this feature, known as library-free search, making DIA analysis more accessible. However, controlling the false discovery rate...
ORGANISM(S): Cellular Organisms 
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
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