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TranscriptomicsGene expression of non-apoptotic pancreatic acinar cell death with autophagy
Serine protease inhibitor Kazal type 3 (Spink3) is a trypsin inhibitor in the pancreas. Spink3-/- pancreatic acinar cells are dead with excessive autophagy at birth (p0.5). To prove the role of nonapoptotic cell death with autophagy, we generated by transgenic technology the pancreas of Spink3-/-;XK...
ORGANISM(S): Mus musculus 
2016-07-16 | E-GEOD-48946 | biostudies-arrayexpress
ProteomicsTMT-based phosphoproteomic analysis of WT and PKD2/3-deficient iNKT cells
Parental and PKD2/3 double-deficient DN32.D3 cells were treated with 10 ug/ml anti-CD3 and cross-linked with 100 ug/ml goat anti-Hamster IgG for 5 min. Cells were washed with ice-cold HEPES-saline and lysed with Guanidine-HCl buffer (6 M guanidine-HCl, 100 mM Tris-Cl pH8.0, 2 mM DTT). The lysates we...
ORGANISM(S): Mus Musculus (mouse) 
2025-09-09 | PXD059288 | JPOST Repository
ProteomicsProximity biotinylation of iNKT cells expressing TurboID-Ikaros with or without TCR stimulation
Ikaros cDNA fused to TurboID was cloned into a retroviral vector and transfected into Phoenix retrovirus packaging cells using PEI MAX (Polysceinces). Culture supernatant containing retroviruses was used for infection into DN32.D3 cells. Cells were cultured in 10%FBS/DMEM which does not contain biot...
ORGANISM(S): Mus Musculus (mouse) 
2025-09-09 | PXD059289 | JPOST Repository
ProteomicsNakaya_VGLL3-FLAG_IP-MS_QEplus
Cardiac myofibroblasts retrovirally transduced with FLAG-VGLL3 or control vector were fixed with 0.1% PFA and lysed in a HEPES-RIPA buffer (20 mM HEPES-NaOH [pH 7.5]/1 mM EGTA/1 mM MgCl2/150 mM NaCl/0.25% sodium deoxycholate/0.05% SDS/1% NP-40) containing protease inhibitors (1:100), phosphatase inh...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039091 | JPOST Repository
ProteomicsImmunnoprecipitation followed by PRM to detect endogenous interaction between VGLL3 and DDX5
For immunoprecipitation to detect the endogenous interaction between VGLL3 and DDX5, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhibi...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039093 | JPOST Repository
ProteomicsImmunoprecipitation followed by PRM to detect endogenous interaction between VGLL3 and EWSR1
For immunoprecipitation to detect the endogenous interaction between VGLL3 and EWSR1, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhib...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039092 | JPOST Repository
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