BioID-based proximity labeling and interactome analysis of the RFFL ubiquitin ligase in CFBE cells expressing WT RFFL-BirA or the catalytically inactive H333A RFFL-BirA mutant.
To characterize the protein interaction network of USP46, BioID analysis was conducted on a knock-in cell line that expresses USP46 and biotin ligase fusion protein stably.