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ProteomicsPrioritization of antimicrobial targets by CRISPR-based oligo recombineering
Nucleophilic amino acids are important in covalent drug development yet underutilized as antimicrobial targets. Over recent years, several chemoproteomic technologies have been developed to mine chemically-accessible residues via their intrinsic reactivity toward electrophilic probes. However, these...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-24 | PXD035658 | Pride
Toxoplasma gondii Oligo recombineering Proteomics 
UnknownMycoplasma pneumoniae Genome Editing Based on Oligo Recombineering and Cas9-Mediated Counterselection.
Not available
S-EPMC7372593 | biostudies-literature
UnknownCRISPR-based oligo recombineering prioritizes apicomplexan cysteines for drug discovery.
Not available
S-EPMC9613468 | biostudies-literature
UnknownEfficient conditional knockout targeting vector construction using co-selection BAC recombineering (CoSBR).
Not available
S-EPMC4627060 | biostudies-literature
UnknownMODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering.
Not available
S-EPMC4086063 | biostudies-literature
UnknownSURE editing: combining oligo-recombineering and programmable insertion/deletion of selection markers to efficiently edit the Mycoplasma pneumoniae genome.
Not available
S-EPMC9825166 | biostudies-literature
UnknownOligonucleotide recombination in Gram-negative bacteria.
Not available
S-EPMC3404488 | biostudies-literature
UnknowntCRISPRi: tunable and reversible, one-step control of gene expression.
Not available
S-EPMC5171832 | biostudies-literature
Genomics
ORBIT for E. coli - mutant library sequencing
PRJNA989253 | ENA
UnknownMycobacterium smegmatis NucS-promoted DNA mismatch repair involves limited resection by a 5'-3' exonuclease and is independent of homologous recombination and NHEJ.
Not available
S-EPMC11551767 | biostudies-literature
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