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TranscriptomicsExperimental analysis of oligonucleotide microarray design criteria to detect deletions by CGH
BACKGROUND: Microarray comparative genomic hybridization (CGH) is currently one of the most powerful techniques to measure DNA copy number in large genomes. In humans, microarray CGH is widely used to assess copy number variants in healthy individuals and copy number aberrations associated with var...
ORGANISM(S): Homo sapiens 
2008-07-26 | E-GEOD-12208 | biostudies-arrayexpress
TranscriptomicsHigh Reproducibility Using Sodium Hydroxide Stripped Long Oligonucleotide DNA Microarrays
Recently, long oligonucleotide (60-70mer) microarrays for two-color experiments have been developed and are gaining widespread use. In addition, when there is limited availability of mRNA from tissue sources, RNA amplification can and is being used to produce sufficient quantities of cRNA for microa...
ORGANISM(S): Homo sapiens 
2010-06-10 | E-GEOD-1706 | biostudies-arrayexpress
TranscriptomicsGene expression profiling in peanut using oligonucleotide microarrays
Transcriptome expression analysis in peanut to date has been limited to a relatively small set of genes and only recently have moderately significant number of ESTs has been released into the public domain. Utilization of these ESTs for the oligonucleotide microarrays provides a means to investigate...
ORGANISM(S): Arachis hypogaea 
2008-12-30 | E-GEOD-11365 | biostudies-arrayexpress
TranscriptomicsImproved detection of global copy number variation using high density, non-polymorphic oligonucleotide probes
Goal: To identify copy number variation in normal individuals using high density, non-polymorphic oligonucleotide probes Background DNA sequence diversity within the human genome may be more greatly affected by copy number variations (CNVs) than single nucleotide polymorphisms (SNPs). Although the ...
ORGANISM(S): Homo sapiens 
2008-04-24 | E-GEOD-9053 | biostudies-arrayexpress
GenomicsOligonucleotide-based arrayCGH
Oligonucleotide-based arrayCGH
PRJNA92921 | ENA
GenomicsDNA-Seq of synthetic DNA from generated library where DNA-arrays being used as “factories” allowing specific DNA oligonucleotide pools
PCR read-off microarrays: The ability to efficiently and economically generate libraries of defined pieces of DNA would have a myriad of applications, not least in the area of defined or directed sequencing and synthetic biology, such as protein engineering and aptamer library screening, but also in...
ORGANISM(S): synthetic DNA 
2011-11-08 | E-MTAB-540 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling with a Blood Pressure QTL interval-specific oligonucleotide array
Although the evidence for a genetic predisposition to human essential hypertension is compelling, the genetic control of blood pressure (BP) is poorly understood. The Dahl salt-sensitive (S) rat is a model for studying the genetic component of BP. Using this model we previously reported the identif...
ORGANISM(S): Rattus norvegicus 
2010-06-30 | E-GEOD-1622 | biostudies-arrayexpress
TranscriptomicsTranscriptional analysis of select CfMNPV genes and some antisense transcripts by an oligonucleotide microarray
The temporal expression of the 23 CfMNPV genes representing all four temporal classes including its 7 unique genes were determined by a modified oligonucleotide-based two-channel DNA microarray. Transcription of the non-coding (antisense) strands of some of the CfMNPV select genes including the poly...
ORGANISM(S): Choristoneura fumiferana MNPV 
2007-05-01 | E-GEOD-4783 | biostudies-arrayexpress
TranscriptomicsTranscript copy number estimation using a mouse whole-genome oligonucleotide microarray (WGA 2.1 Spike Test)
E12.5 mouse whole embryo, E12.5 placenta, embryonic stem (ES) cells, and trophoblast stem (TS) cells were compared on a novel microarray design to validate the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 44,000 DNA features, and was designed to ...
ORGANISM(S): Mus musculus 
2005-10-28 | E-GEOD-3509 | biostudies-arrayexpress
GenomicsLegionella ITS oligonucleotide microarray
Legionella ITS oligonucleotide microarray
PRJNA263045 | ENA
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