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H3K27ac and H4K5ac ChIPseq in WT and Hdac3-/- mESCs in order to determine the role of Hdac3 on the activity of cis-regulatory elements during differentiation.
This experiment is part of the study: "Mechanistic framework of lineage restriction in embryonic stem cells" by Olivieri et al.
ORGANISM(S): Mus musculus 
ATACseq in WT, Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones of mESCs in order to determine the role of the Dax1/Nr5a2 complex on chromatin accessibility and the activity of cis-regulatory elements during differentiation.
This experiment is part of the study: "Mechanistic framework of lineage rest...
ORGANISM(S): Mus musculus 
Determine the transcriptomic effects that are associated with the knock-out of the inhibitor of PrE trans-differentiation Dax1 and the associated nuclear receptor Nr5a2.
This experiment is part of the study: "Mechanistic framework of lineage restriction in embryonic stem cells" by Olivieri et al.
ORGANISM(S): Mus musculus 
Determine the transcriptomic effects that are associated with the distinct differentiation phenotypes of HDAC3 knock-out vs wildtype mESCs.
This experiment is part of the study: "Mechanistic framework of lineage restriction in embryonic stem cells" by Olivieri et al.
ORGANISM(S): Mus musculus 
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