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GenomicsHistone Demethylase Lsd1 is Required to Repress Hematopoietic Stem and Progenitor Cell Signatures During Blood Cell Maturation
Here we describe that lysine-specific demethylase 1 (Lsd1/KDM1a), which demethylates histone H3 on LysM-bM-^@M-^I4 or LysM-bM-^@M-^I9 (H3K4/K9), is an indispensible epigenetic governor of hematopoietic differentiation. Integrative genomic analysis in primary hematopoietic cells, combining global occ...
ORGANISM(S): Mus musculus 
2013-06-25 | E-GEOD-40440 | biostudies-arrayexpress
TranscriptomicsExpression data from BCL11A-null and wild-type bone marrow erythroid pregenitors
BCL11A is a critical mediator of hemoglobin switching and gamma-globin silencing. In this study, we showed the BCL11A is required in vivo for developmental silencing of gamma-globin genes in adult animals. We used microarray to determine the changes in gene expression profile after loss of BCL11A in...
ORGANISM(S): Mus musculus 
2012-03-22 | E-GEOD-28333 | biostudies-arrayexpress
TranscriptomicsGene expression data of Lsd1fl/fl and Lsd1fl/fl Mx1Cre Gr1dim Mac1 granulocytic progenitor cells.
We discovered that mice with hematopoietic-specific deletion of Lsd1 lacked Gr-1+ Mac1+ neutrophilic granulocytes whereas the numbers of Gr-1dim Mac1+ granulocytic progenitor cells was increased. To determine the genes altered by Lsd1-loss, Gr-1dim Mac1+ granulocytic progenitor cells from Lsd1fl/fl ...
ORGANISM(S): Mus musculus 
2013-06-25 | E-GEOD-40282 | biostudies-arrayexpress
TranscriptomicsGene expression data of Lsd1fl/fl and Lsd1fl/fl Mx1Cre CD150+ CD48- lin- c-Kit+ Sca-1+ LT-HSCs
We discovered that mice that lack Lsd1 in hematopoietic cells were exhibited increased frequencies of CD150+ CD48- lin- c-Kit+ Sca-1+ LT-HSCs, but completely lacked the lin- c-Kit+ Sca-1- myeloid progenitor compartment. To determine the genes altered by Lsd1-loss, CD150+ CD48- lin- c-Kit+ Sca-1+ LT-...
ORGANISM(S): Mus musculus 
2013-06-25 | E-GEOD-40284 | biostudies-arrayexpress
TranscriptomicsExpression data for conditional Dnmt1knockout hematopoietic stem and progenitor cells
DNA methylation is essential for mammalian development and plays crucial roles in a variety of biological processes. The DNA methyltransferase Dnmt1 serves to maintain parental cell methylation patterns on daughter DNA strands in mitotic cells, however, the precise role of Dnmt1 in regulation of qui...
ORGANISM(S): Mus musculus 
2010-03-03 | E-GEOD-15840 | biostudies-arrayexpress
TranscriptomicsMicroarray profiling analysis in Jmj-Fl/Fl and Jmj-null ESCs.
We used microarrays to detail the role of JMJ in ES cell function. Keywords: cell type and time course Cells were harvested for RNA extraction and hybridization on Affymetrix microarrays.
ORGANISM(S): Mus musculus 
2009-12-29 | E-GEOD-19165 | biostudies-arrayexpress
TranscriptomicsEHMT1 and EHMT2 inhibition induce fetal hemoglobin expression [RNA-seq]
Using UNC0638 and genetic assays to inhibit EHMT1/2 and derepress fetal hemoglobin in adult hematopoietic cells. RNA-Seq in primary adult human erythroid cells treated with UNC0638 or the vehicle control (DMSO) in biological triplicates.
ORGANISM(S): Homo sapiens 
2015-11-02 | E-GEOD-71421 | biostudies-arrayexpress
GenomicsComparative profiling of chromatin state maps and transcription factor occupancy during human fetal and adult erythropoiesis
We report the comparative investigation of genome-wide chromatin state maps, transcription factor (TF) occupancy, and gene expression profiles from developing red cell precursors at two developmental stages. Contrasting the similarities and differences between fetal and adult erythropoiesis provides...
ORGANISM(S): Homo sapiens 
2012-10-05 | E-GEOD-36985 | biostudies-arrayexpress
GenomicsBCL11A cistrome in mouse erythroleukemia (MEL) cells
ChIP-seq analyses were performed in MEL cells expressing BirA alone or BirA and FLAG-Biotin tagged BCL11A (XL isoform). BCL11A chromatin occupancy in MEL cell line.
ORGANISM(S): Mus musculus 
2014-04-01 | E-GEOD-28334 | biostudies-arrayexpress
GenomicsScl specifies hemogenic endothelium and inhibits cardiogenesis via primed enhancers [ChIP-seq]
Scl/Tal1 confers hemogenic competence and prevents cardiomyogenesis in embryonic endothelium. Here we show that Scl both directly activates a broad gene regulatory network required for hematopoietic stem/progenitor cell (HS/PC) development, and represses transcriptional regulators required for cardi...
ORGANISM(S): Mus musculus 
2015-01-01 | E-GEOD-47082 | biostudies-arrayexpress
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