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GenomicsChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing
We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein. One replicate is incl...
ORGANISM(S): Drosophila melanogaster 
2011-10-18 | E-GEOD-31899 | biostudies-arrayexpress
TranscriptomicsExpression profile of third instar larval fat body and midgut tissues
We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Fat bodies from wandering third instar larvae were dissected from ~50 male ...
ORGANISM(S): Drosophila melanogaster 
2010-11-02 | E-GEOD-25025 | biostudies-arrayexpress
TranscriptomicsmicroRNA (miRNA) expression profiles of murine polyploid trophoblast giant cells (TGCs) from day 9.5 implantation sites.
We employed miRNA-seq to profile all miRNAs from a pure population of hand-dissected polyploid TGCs from embryonic day 9.5. These data set of polyploid-specific TGCs microRNAs will provide insights into TGCs differentiation and endoreplication. TGCs were micro-dissected from day E9.5 nine implantati...
ORGANISM(S): Mus musculus 
2013-04-30 | E-GEOD-45784 | biostudies-arrayexpress
TranscriptomicsRNA expression profiles of murine polyploid trophoblast giant cells (TGCs) from day 9.5 implantation sites.
We employed RNA-seq to transcriptionally profile a pure population of hand-dissected polyploid TGCs from embryonic day 9.5. These data provide a set of polyploid-specific TGCs transcripts that will aid in the understanding of TGCs differentiation and endoreplication. TGCs were micro-dissected from d...
ORGANISM(S): Mus musculus 
2013-04-30 | E-GEOD-45782 | biostudies-arrayexpress
TranscriptomicsExpression profile of third instar larval salivary gland tissue
We use mRNA-seq to transcriptionally profile larval salivary gland tissue from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Salivary glands were dissected from 200 wandering third instar larvae and the ass...
ORGANISM(S): Drosophila melanogaster 
2011-10-18 | E-GEOD-33017 | biostudies-arrayexpress
TranscriptomicsExpression profile of 16C ovarian follicle cells
We use DSN normalized RNA-seq to transcriptionally profile FACS sorted 16C ovarian follicle cells. These data provide insights into the developmental control of gene expression programmed gene amplificaton. Follicle cells were isolated from whole ovaries by trypsinization and filtering and stained w...
ORGANISM(S): Drosophila melanogaster 
2011-05-27 | E-GEOD-29526 | biostudies-arrayexpress
TranscriptomicsSMAUG Is a Major Regulator of Maternal mRNA Destabilization in Drosophila
In animals, egg activation triggers a cascade of posttranscriptional events that act on maternally synthesized RNAs. We show that, in Drosophila, the PAN GU (PNG) kinase sits near the top of this cascade, triggering translation of SMAUG (SMG), a multifunctional posttranscriptional regulator conserve...
ORGANISM(S): Drosophila melanogaster 
2007-09-13 | E-GEOD-8910 | biostudies-arrayexpress
TranscriptomicsPolysome profiling and ribosome footprinting of Drosophila mature oocyte and activated egg
We use mRNA-seq in combination with polysome profiling to determine translational status for all mRNAs in Drosophila mature oocytes and activated eggs. Puromycin-treated lysates are used as a negative control in polysome profiling experiments. Additionally, we use ribosome footprinting to globally m...
ORGANISM(S): Drosophila melanogaster 
2014-06-12 | E-GEOD-52799 | biostudies-arrayexpress
GenomicsEarly Origins Meta-peak generation
modENCODE_submission_3441 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known replication in...
ORGANISM(S): Drosophila melanogaster 
2011-03-16 | E-GEOD-27981 | biostudies-arrayexpress
TranscriptomicsMidgut - CGH Differential Replication
modENCODE_submission_3278 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known ...
ORGANISM(S): Drosophila melanogaster 
2011-03-25 | E-GEOD-28179 | biostudies-arrayexpress
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