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TranscriptomicsDeficiency of CXCR2, but Not Other Chemokine Receptors, Attenuates Autoantibody-Mediated Arthritis in a Murine Model
To examine patterns of gene expression in ankle synovial fluid cells and peripheral blood leukocytes during serum transferred arthritis. A time-course microarray analysis of serum-transferred arthritis was performed, examining ankle tissue, synovial fluid, and peripheral blood leukocytes.
ORGANISM(S): Mus musculus 
2011-02-24 | E-GEOD-27492 | biostudies-arrayexpress
TranscriptomicsCell specific response to IFNa
We report cell specific responses to IFNa in 11 different peripheral immunocyte populations in the mouse. These cells represent the core ImmGen immunocyte lineage panel. Profiles from these were used to analyze cell specific responses to IFNa. In general a core set of ISG transcripts are induced in ...
ORGANISM(S): Mus musculus 
2015-11-20 | E-GEOD-75202 | biostudies-arrayexpress
TranscriptomicsKinetics of IFNa response
We describe finely resolved kinetics of the transcriptional response to IFNa in B cells in vivo. Temporal changes in expression of the most robustly induced ISGs were analyzed across 23 (15min - 15hrs) timepoints. Most ISGs reach peak induction at approximately 2hrs and generally in a synchronus ma...
ORGANISM(S): Mus musculus 
2015-11-20 | E-GEOD-75195 | biostudies-arrayexpress
TranscriptomicsIFNa dose response in primary murine B cells
B cells respond robustly to IFNa. Here we analyze gene expression profiles of primary murine splenic B cells treated with 10 fold serially diluted IFNa in vitro. We explore sensitivity to ISGs to IFNa as they relate to dose. Generally ISGs do not cluster significantly in a dose dependent manner. How...
ORGANISM(S): Mus musculus 
2015-11-20 | E-GEOD-75194 | biostudies-arrayexpress
TranscriptomicsDependency of ISG expression on IFNAR1 and Tyk2
We analyze the expression profile of ISGs in the context of IFNAR1-KO primary murine B cells and macrophages. These analses were used to define ISG gene sets that are under tonic control. Furthermore, these analyses enabled the definition of ISGs that are dependent on Tyk2 signaling. Male C57BL/6 IF...
ORGANISM(S): Mus musculus 
2015-11-20 | E-GEOD-75203 | biostudies-arrayexpress
TranscriptomicsEffect of JAK blockade on IFNa response in B cells in vitro
B cells respond robustly to type 1 interferons which signal through JAK1 and TYK2. Here we analyzed the effects of a panel of JAK inhibitors on the IFNa transcriptional response in activated B cells in vitro. Splenic B cells were isolated by negative selection from 6wk old male C57BL/6 mice. These w...
ORGANISM(S): Mus musculus 
2016-07-20 | E-GEOD-84560 | biostudies-arrayexpress
TranscriptomicsEffect of JAK blockade on IFNa response in CD4+ T cells in vitro
CD4+ T cells respond robustly to type 1 interferons which signal through JAK1 and TYK2. Here we analyzed the effects of a panel of JAK inhibitors on the IFNa transcriptional response in activated CD4+ T cells in vitro. Splenic CD4+ T cells were isolated by negative selection from 6wk old male C57BL...
ORGANISM(S): Mus musculus 
2016-07-20 | E-GEOD-84565 | biostudies-arrayexpress
TranscriptomicsImmuno-genomic effects of JAK blockade in vivo
Small molecule inhibitors of JAK kinases have shown clinical effcacy in the treatment of certain autoimmune diseases. While these are known to block upstream JAK signalling events, their broader impact on the transcriptional footprint in immunocytes are unknown. Here we explore the effects of pan- a...
ORGANISM(S): Mus musculus 
2016-07-27 | E-GEOD-84853 | biostudies-arrayexpress
TranscriptomicsB cell response to IFNa in vitro
Here we analyzed thetranscriptional response to IFNa in activated B cells in vitro. We found robust induction of ISGs as early as 2hrs. Splenic B cells were isolated by negative selection from 6wk old male C57BL/6 mice. These were activated with LPS(10ug/ml) for 48hrs and thereafter cultured with IF...
ORGANISM(S): Mus musculus 
2016-07-20 | E-GEOD-84561 | biostudies-arrayexpress
TranscriptomicsCD4+ T cell response to IL12 in vitro
Here we analyzed the transcriptional response to IL12 in activated CD4+ T cells in vitro. Splenic CD4+ T cells were isolated by negative selection from 6wk old male C57BL/6 mice. These were activated with CD3/28 beads for 48hrs and thereafter cultured with IL12 (100U/ml) for 2hrs or 8hrs in vitro. C...
ORGANISM(S): Mus musculus 
2016-07-20 | E-GEOD-84567 | biostudies-arrayexpress
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