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ProteomicsLimited proteolysis for PTM analysis
PTMs on a mycobacterial DNA-binding protein with a long, lysine-rich, intrinsically disorders region were analyzed by limited proteolysis with trypsin, unveiling a massive methylation of most lysine residues of the protein in quantitative manner, which was lacked in the recombinant version of the pr...
ORGANISM(S): Escherichia Coli Mycobacterium Tuberculosis Mycolicibacterium Smegmatis Mc2 155 
2024-06-14 | PXD042969 | JPOST Repository
ProteomicsMS analysis of BCG and recombinant BCG "70C-RBD"
BCG and recombinant BCG "70C-RBD" were cultured in Sauton media. Culture filtrate and cells lysate were separated by SDS-PAGE. The bands at 40kDa and 60kDa were excised and proteins were reduced and alkylated. In-gel digestion (Trypsin) was performed. The samples were analyzed by TripleTOF5600+ conj...
ORGANISM(S): Cellular Organisms 
2023-12-07 | PXD046929 | JPOST Repository
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