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ProteomicsPRM quantification of XRCC4 and Xkr4 peptides that were immunoprecipitated with SPOT-Xkr4-Flag
Several selected peptides of human XRCC4 and mouse Xkr4 were measured by PRM. LC-MS/MS analysis was performed on an EASY-nLC 1200 UHPLC connected to a Q Exactive Plus mass spectrometer through a nanoelectrospray ion source (Thermo Fisher Scientific). Targeted MS/MS scans were acquired by a time-sche...
ORGANISM(S): Homo Sapiens (human) 
2021-01-12 | PXD023537 | JPOST Repository
ProteomicsLabel-free quantification of Xkr4 interactors
Membrane fractions of XRCC4 KO PLB cells expressing SPOT-Xkr4-FLAG and XRCC4 (WT or R270A) were solubilized by detergent and immunoprecipitated with anti-SPOT nanobody-conjugated beads to precipitate Xkr4 interactors, followed by mass spectrometry.
ORGANISM(S): Homo Sapiens (human) 
2021-01-12 | PXD023536 | JPOST Repository
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