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ProteomicsMudPIT analysis of S. pombe proteins interacting with Pof8
To identify proteins interacting with Pof8, cell-free extracts of strains FP1547 harboring 3xFLAG epitope-tagged Pof8 and untagged control (FP1546) were diluted to 5 mg/ml in 8 ml of lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM sodium chloride, 0.1% (v/v) NP40 and 10% (v/v) glycerol) plus complete ED...
ORGANISM(S): Schizosaccharomyces Pombe (ncbitaxon:4896) 
2021-05-24 | MSV000087493 | MassIVE
TranscriptomicsHost gene expression profiling on children with any cause critical illness at retrieval or admission to paediatric critical care
We analysed data collected as part of the Biomarkers of Acute Serious Illness in Children (BASIC) study. BASIC was a prospective cohort study that enrolled critically ill children admitted to four PICUs in London and Eastern England, UK, during emergency transport by the Children’s Acute Transport S...
ORGANISM(S): Homo sapiens 
2025-09-30 | E-MTAB-15164 | biostudies-arrayexpress
GenomicsSystematic identification of G-quadruplex sensitive lethality by genome-wide genetic screening
A375 and HT1080 cells are treated with G-quadruplex ligands PDS or PhenDC3 following genome-wide shRNA knockdown. This enables the identification of genes that when silenced, specifically compromises cell growth in the presence of the ligand. First, a pilot screen was performed to determine a ligand...
ORGANISM(S): Homo sapiens 
2019-06-22 | E-MTAB-6367 | biostudies-arrayexpress
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