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ProteomicsHuman induced pluripotent stem cell-derived cardiomyocyte secretome
hiPSC-CM heavy water labeling calibration standard AICS-0052-003 hiPSC (mono-allelic C-terminus mEGFP-tagged MYL7 WTC-11; Allen Institute Cell Collection) line was acquired from Coriell Institute and seeded onto Geltrex (Gibco) coated 6 well plates and maintained in mTesR Plus basal media with Supp...
ORGANISM(S): Homo Sapiens (human) 
2025-07-24 | PXD060295 | JPOST Repository
ProteomicsHuman AC16 Cardiac Cells under Doxorubicin Treatment
AC16 acquired from Millipore and cultured in DMEM/F12 with 10% FBS. For the mass spectrometry experiment, the AC16 cells were switched to Fibroblast Growth Medium (FGM-3) with supplements (Promocell) then treated with 0.1 µM doxorubicin for 24 hours, then co-cultured through non-contact transwells w...
ORGANISM(S): Homo Sapiens (human) 
2023-04-22 | PXD041722 | JPOST Repository
ProteomicsHuman induced pluripotent stem cells under dynamic heavy water labeling
Fractional synthesis calibration cells for hiPSC AICS-0052-003 hiPSC (mono-allelic C-terminus mEGFP-tagged MYL7 WTC-11; Allen Institute Cell Collection) line was acquired from Coriell Institute and seeded onto Geltrex (Gibco) coated 6 well plates and maintained in mTesR Plus basal media with Supplem...
ORGANISM(S): Homo Sapiens (human) 
2025-07-23 | PXD060287 | JPOST Repository
ProteomicsCalibration and heavy water labeling in normal and stressed human AC16 cells
For calibration curve samples, human AC16 cells (Millipore) were cultured in DMEM/F12 supplemented with 10% FBS and either 6% D2O (heavy labeled population) or 6% H2O (control population) at 37°C, 5% CO2. The cells were maintained in this medium for 3 passages, each passage with a split ratio of 1:8...
ORGANISM(S): Homo Sapiens (human) 
2025-07-23 | PXD048321 | JPOST Repository
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