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ProteomicsIn vitro phosphorylation of trypsin-digested K562 cell lysate by TAM family kinases
K562 cell lysate was digested with trypsin, dephosphorylated with lambda phosphatase, and treated in an in vitro kinase reaction with Axl, Mer or Tyro3 kinase, alongside a control reaction for each that contained no kinase. Samples were phosphoenriched with the SMOAC approach (TiO2 and FeNTA) and an...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-29 | MSV000092784 | MassIVE
ProteomicsNovel Brutons Tyrosine Kinase (BTK) substrates for time-resolved luminescence assays
BTK substrate profile determined by treating digest KG-1 cell lysate with alkaline phosphatase followed by recombinant BTK. Samples were enriched with PolyMac phosphoenrichment kits prior to analysis. We have organized our uploaded files into three main folders: 1. BTK KALIP MS raw files: raw mass ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-11-22 | MSV000088429 | MassIVE
ProteomicsHigh-throughput identification of FLT3 wild-type and mutant kinase substrate preferences and application to design of sensitive in vitro kinase assay substrates
Abnormalities in the FLT3 signaling pathway play an integral role in AML disease relapse and drug resistance. Developing new and specific FLT3 tyrosine kinase inhibitors for use in combination to induction therapy is an important step to reduce disease relapse and achieve clinical remission. To deve...
ORGANISM(S): Homo sapiens (Human) 
2019-01-07 | PXD011478 | Pride
Kinase assay linked with phosphoproteomics Flt3 Acute myeloid leukemia Mass spectrometry Phosphoproteomics 
UnknownDe novo mutations across 1,465 diverse genomes reveal mutational insights and reductions in the Amish founder population.
Not available
S-EPMC7007577 | biostudies-literature
UnknownGuidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Not available
S-EPMC4835977 | biostudies-literature
UnknownDeath following pulmonary complications of surgery before and during the SARS-CoV-2 pandemic.
Not available
S-EPMC10364875 | biostudies-literature
UnknownPreliminary model assessing the cost-effectiveness of preoperative chlorhexidine mouthwash at reducing postoperative pneumonia among abdominal surgery patients in South Africa.
Not available
S-EPMC8360544 | biostudies-literature
UnknownA prognostic model for use before elective surgery to estimate the risk of postoperative pulmonary complications (GSU-Pulmonary Score): a development and validation study in three international cohorts.
Not available
S-EPMC12056793 | biostudies-literature
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