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TranscriptomicsTranscripts induced by exposure to SLE serum in healthy blood monocytes
Monocytes from 3 healthy donors were cultured for 6 hours in the presence of 20% serum from three newly diagnosed, untreated SLE patients. Microarray analysis was then performed upon normalizing the gene expression levels of samples incubated with SLE sera to those incubated with autologous serum. M...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-46920 | biostudies-arrayexpress
TranscriptomicsTranscripts differentially expressed in healthy blood mDCs compared to healthy monocytes
To directly compare the SLE monocyte transcriptional program with that of blood mDC precursors, we purified lineage HLA-DRhighCD11chigh mDCs and CD14+ monocytes from the blood of five healthy donors. Their gene expression profiles were then compared to those of blood SLE monocytes. An unsupervised c...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-46913 | biostudies-arrayexpress
TranscriptomicsDifferentially expressed transcripts in SLE blood monocytes according to their capacity to induce mixed lymphocytic reaction
We screened SLE monocytes from 19 SLE patients and selected 4 that induced CD4+ T cell proliferation in vitro and 4 that did not. CFSE labeled CD4-T cells (105) were incubated with SLE monocytes (2 x 104). Cells were harvested at 6 hours for RNA extraction. We screened SLE monocytes from 19 SLE pati...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-46917 | biostudies-arrayexpress
TranscriptomicsSLE serum induced transcripts regulated by IFNa2b
To explore the full extent of IFN-regulated transcriptional changes, we exposed monocytes from two healthy donors to recombinant type I IFN (IFN-M-NM-12b) in vitro. RNA was extracted at 6 hrs and the expression data was normalized to that of monocytes cultured with medium. Blood monocytes isolated f...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-46921 | biostudies-arrayexpress
TranscriptomicsDifferentially expressed transcripts in SLE blood monocytes
To better characterize the molecules that could potentially confer antigen presenting capacity to SLE monocytes, we assessed their gene expression profile. Blood monocytes from five healthy controls and five pediatric SLE patients were isolated using CD14+ selection. Because drugs used to treat SLE ...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-46907 | biostudies-arrayexpress
TranscriptomicsTranscripts induced by recombinant IFNa in healthy blood monocytes at different time points
To explore the full extent of IFN-regulated transcriptional changes, we exposed monocytes from two healthy donors to recombinant type I IFN (IFN-M-NM-12b) in vitro. RNA was extracted at different incubation times (1, 6, 24, 48 and 72 hrs) and the expression data was normalized to that of monocytes c...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-46911 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of blood, spleen and lung expression of C57BL/6 mice subjected to Burkholderia pseudomallei infection
Melioidosis, a severe human disease caused by the bacterium Burkholderia pseudomallei, has a wide spectrum of clinical manifestations ranging from acute septicaemia to chronic localized illness or latent infection. Mice were intranasally infected with either high or low doses of B. pseudomallei to g...
ORGANISM(S): Mus musculus 
2015-09-02 | E-GEOD-61106 | biostudies-arrayexpress
TranscriptomicsBlood Leukocyte Microarrays to Diagnose Systemic Onset Juvenile Idiopathic Arthritis and Follow IL-1 blocade
Systemic onset Juvenile Idiopathic Arthritis (SoJIA) represents up to 20% of Juvenile Idiopathic Arthritis (JIA). We have previously reported that this disease is Interleukin 1 (IL1)-mediated, and that IL-1 blockade results in clinical remission in the majority of patients. The diagnosis of SoJIA, h...
ORGANISM(S): Homo sapiens 
2007-09-01 | E-GEOD-8650 | biostudies-arrayexpress
TranscriptomicsTrivalent Inactivated Influenza Vaccine (TIV) and Live Attenuated Influenza Vaccine (LAIV) Induce Different B cell and Transcriptional Responses in Children
LAIV and TIV are effective for prevention of influenza infection in children, but the mechanisms associated with protection are still not well defined. We analyzed the differences in B cell responses and transcriptional profiles. Compared to baseline, LAIV elicited a significant increase in naM-CM-/...
ORGANISM(S): Homo sapiens 
2014-03-31 | E-GEOD-52005 | biostudies-arrayexpress
TranscriptomicsGenomic Fingerprints of DC Subsets in the Human Vaginal Mucosa, Skin, and Blood
Dendritic cells (DC) localize throughout the body, where they sense and capture invading pathogens to induce protective immune responses. Hence, harnessing the biology of tissue-resident DC is crucial for the rational design of vaccines against pathogens. Herein, we characterized the transcriptomes ...
ORGANISM(S): Homo sapiens 
2014-12-17 | E-GEOD-54480 | biostudies-arrayexpress
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