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GenomicsGenome wide mapping of Jarid2 and Suz12 binding sites in mouse ES cells before and after Jarid2 depletion
We analyzed the overlap in genome wide binding sites between Jarid2 and Suz12 in mouse ES cells and find that Jarid2 and Suz12 peaks have an high degree (90%) of overlap. Moreover we analyzed the effect of Jarid2 down regulation on genome wide Suz12 binding sites and found that Loss of Jarid2 lead t...
ORGANISM(S): Mus musculus 
2010-01-27 | E-GEOD-19365 | biostudies-arrayexpress
TranscriptomicsExpression changes in Yy1 knock down mouse embryonic stem cells
We have determined the global gene expression upon loss of function of the Yy1 transcription factor in mouse embryonic stem cells Total RNA was extracted from ES cells transiently transfected with yy1-specific or scrampled-control siRNA oligos 48 hr post-transfection.
ORGANISM(S): Mus musculus 
2012-01-04 | E-GEOD-31784 | biostudies-arrayexpress
GenomicsYy1 activity in mouse embryonic stem cells
This SuperSeries is composed of the following subset Series: GSE31784: Expression changes in Yy1 knock down mouse embryonic stem cells GSE31785: Yy1 occupancy of mouse ES cell genome Refer to individual Series
ORGANISM(S): Mus musculus 
2012-01-04 | E-GEOD-31786 | biostudies-arrayexpress
GenomicsYy1 occupancy of mouse ES cell genome
These data include the genome wide location analyses of Yy1 by bioChIPs of Fbio-Yy1. Streptavidin precipitation of formaldehyde cross-linked chromatin prepared from Fbio-Yy1 and control expressing mouse ES cells
ORGANISM(S): Mus musculus 
2012-01-04 | E-GEOD-31785 | biostudies-arrayexpress
TranscriptomicsThe Polycomb Group Protein Suz12 Is Required for Embryonic Stem Cell Differentiation
Polycomb group (PcG) proteins form multiprotein complexes, called Polycomb repressive complexes (PRCs). PRC2 contains the PcG proteins EZH2, SUZ12, and EED and represses transcription through methylation of lysine (K) 27 of histone H3 (H3). Suz12 is essential for PRC2 activity and its inactivation r...
ORGANISM(S): Mus musculus 
2011-08-11 | E-GEOD-31354 | biostudies-arrayexpress
GenomicsPolycomb-dependent H3K27me1 and H3K27me2 characterization in mouse ES cells (E36 with H3K27ac, H3K27me1, and H3K4me1/3)
These data include the genome wide location analysis of H3K27me1/2/ac and H3K4me1/3 by ChIP in mouse ES cells. Precipitation of formaldehyde cross-linked chromatin prepared from mouse ES cell using specific antibodies against Histone H3 and its specific modifications. Rabbit IgG were used as contro...
ORGANISM(S): Mus musculus 
2013-12-05 | E-GEOD-39496 | biostudies-arrayexpress
GenomicsMaintenance of Leukemic Cell Identity by the Activity of the Polycomb Complex PRC1 (ChIP-seq)
These data include the genome wide occupancy of H2AUbq by ChIP sequencing in Cdkn2a-/- Lin- HSC cells non-transduced or transduced with MLL-AF9, HOXA9 and PML-RARa. Immuno-precipitation of formaldehyde cross-linked chromatin prepared from Cdkn2a-/- Lin- HSC cells non-transduced or transduced with ML...
ORGANISM(S): Mus musculus 
2016-10-21 | E-GEOD-67498 | biostudies-arrayexpress
TranscriptomicsMaintenance of Leukemic Cell Identity by the Activity of the Polycomb Complex PRC1 (RNA-seq)
These data include RNA Seq data generated from Ring1b wild type and Ring1b KO Ring1a-/- Cdkn2a-/- Lin- HSC cells non-transduced or transduced with MLL-AF9, HOXA9 and PML-RARa. Total RNA extracted from Ring1b wild type and Ring1b KO Ring1a-/- Cdkn2a-/- Lin- HSC cells non-transduced or transduced wit...
ORGANISM(S): Mus musculus 
2016-10-21 | E-GEOD-67517 | biostudies-arrayexpress
TranscriptomicsExpression data from wild type, Ring1B-/-, Eed-/-, and Ring1B/Eed double deficient mouse ES cells
Polycomb complexes establish chromatin modifications for maintaining gene repression and are essential for embryonic development in mice. Here we use pluripotent embryonic stem (ES) cells to demonstrate an unexpected redundancy between Polycomb repressive complex 1 (PRC1) and PRC2 during the formati...
ORGANISM(S): Mus musculus 
2009-11-30 | E-GEOD-19076 | biostudies-arrayexpress
GenomicsPolycomb-dependent H3K27me1 and H3K27me2 regulate active transcription and enhancer fidelity
These data include the genome wide location of different histone modifications by ChIP sequencing in mouse ES cells, and RNA Seq data generated from wild type and EED KO mouse ES cells and knocked down for unrelated protein and Setd2 protein. ChIP-Seq: Immuno-precipitation of formaldehyde cross-lin...
ORGANISM(S): Mus musculus 
2013-12-05 | E-GEOD-51006 | biostudies-arrayexpress
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