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GenomicsRole of Polycomb group proteins in the DNA damage response – a reassessment
We have investigated whether components of Polycomb repressive complex 1 (PRC1) are recruited to double-strand breaks (DSBs) generated by inducible expression of the AsiSI restriction enzyme in normal human fibroblasts. Using chromatin immunoprecipitation and deep sequencing (ChIP-seq), which detec...
ORGANISM(S): Homo sapiens 
2014-07-06 | E-GEOD-55605 | biostudies-arrayexpress
GenomicsThe Scc2NIPBL/Scc4MAU2 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions [ChIP-seq]
The Scc2/Scc4 complex binds to broad nucleosome-free regions in the promoters of highly expressed genes. The cohesin loader is recruited to these sites by the RSC chromatin remodeling complex ChIp- Sequencing analysis of the distribution of Scc2 and Scc4 on budding yeast chromosomes.An input sample ...
ORGANISM(S): Saccharomyces cerevisiae 
2014-08-01 | E-GEOD-56993 | biostudies-arrayexpress
GenomicsThe Scc2NIPBL/Scc4MAU2 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions [Mnase-seq]
The Scc2/Scc4 complex binds to broad nucleosome-free regions in the promoters of highly expressed genes. The cohesin loader is recruited to these sites by the RSC chromatin remodeling complex MNase digestion of purified DNA followed by paired-end sequencing was performed in order to investigate geno...
ORGANISM(S): Saccharomyces cerevisiae 
2014-08-01 | E-GEOD-56992 | biostudies-arrayexpress
TranscriptomicsThe Scc2NIPBL/Scc4MAU2 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions [microarray]
The Scc2/Scc4 complex binds to broad nucleosome-free regions in the promoters of highly expressed genes. The cohesin loader is recruited to these sites by the RSC chromatin remodeling complex Total RNA was isolated from wt, scc2-4 and sth1-3 cells at 25°C and 37°C. dscDNA was obtained using the IV...
ORGANISM(S): Saccharomyces cerevisiae 
2014-08-01 | E-GEOD-56991 | biostudies-arrayexpress
GenomicsGenome wide co-localization of Polycomb orthologs and their effects on gene expression in human fibroblasts
We have identfied antibodies that support chromatin immunoprecipitation (ChIP) of 11 components of Polycomb repressive complex 1 (PRC1) and shown that multiple variants of PRC1 associate with the same DNA. By performing ChIP-seq with antibodies against CBX6, CBX7, CBX8, RING1 and RING2, in two stra...
ORGANISM(S): Homo sapiens 
2013-12-19 | E-GEOD-40740 | biostudies-arrayexpress
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